Chicken Arginine vasopressin (AVP) ELISA Kit | AVP elisa kit

Chicken Arginine vasopressin (AVP) ELISA Kit

Cat. Num. AAA267027

• Reactivity: Chicken
• Synonyms: Arginine vasopressin (AVP); Chicken Arginine vasopressin (AVP) ELISA Kit; AVP elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 17

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: Up to 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for AVP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken AVP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 10334810
• Molecular Weight: 1,923 Da
• NCBI Official Full Name: arginine vasopressin, partial
• UniProt Protein Name: Arginine vasopressin
• Protein Family: Neurophysin
• UniProt Gene Name: AVP
• UniProt Entry Name: Q9HB76_HUMAN

NCBI Description

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Uniprot Description

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule

Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; protein kinase activity; receptor binding

Biological Process: circadian rhythm; response to nicotine; water transport; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; positive regulation of systemic arterial blood pressure; locomotory behavior; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; cell-cell signaling; hyperosmotic salinity response; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; transmembrane transport; positive regulation of prostaglandin biosynthetic process; penile erection; grooming behavior; vasoconstriction; sodium-independent organic anion transport; generation of precursor metabolites and energy; maternal behavior; response to testosterone stimulus; social behavior; positive regulation of peptidyl-serine phosphorylation; positive regulation of cell growth; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis

Disease: Diabetes Insipidus, Neurohypophyseal