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Testing Data

General GAPDH II Assay Kit | GAPDHII assay kit

Human GAPDH Activity Assay Kit II

Reactivity
General
Synonyms
GAPDH II; Human GAPDH Activity Assay Kit II; GAPDHII assay kit
Ordering
For Research Use Only!
Reactivity
General
Specificity
Measurement of GAPDH activity in various tissues and cells; Analysis of glycolysis and pentose phosphate pathways
Detection Method
Absorbance (OD 450nM)
Sample Type
Animal tissues: Liver, Heart etc., Cell culture: Adherent or suspension cells
Features & Benefits
Sensitive method for monitoring GAPDH activity in various sample types. Simple, rapid & convenient
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months

Testing Data

Testing Data
Related Product Information for GAPDHII assay kit
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH is a stable metabolic enzyme and is constitutively expressed. Therefore, it is considered as a housekeeping gene. Deregulation of GAPDH activity is associated with cell proliferation and carcinogenesis. Accurate detection of GAPDH activity is important for testing diseases and studying normal cellular physiology. Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450nM. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.
Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450nM. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.

Similar Products

Product Notes

The General GAPDHII (Catalog #AAA8400629) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400629 Assay Kit recognizes General GAPDHII. It is sometimes possible for the material contained within the vial of "GAPDH II, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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