Western Blot (WB)
(Western blot shows lysates of mouse thymus tissue and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1ug/ml of Sheep Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Themis at approximately 73kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Sheep anti-Mouse, Rat Themis Polyclonal Antibody
Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection)
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Themis; Polyclonal Antibody; Themis (Protein THEMIS; Grb2-associating Protein; Gasp; Protein Thylex; Thymocyte-expressed Molecule Involved in Selection); Anti -Themis (Protein THEMIS; anti-Themis antibody
Host
Sheep
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Themis. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Applicable Applications for anti-Themis antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml
Dilution: Western Blot: 1ug/ml
Immunogen
Recombinant corresponding to aa122-237 from mouse Themis expressed in E.coli.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot shows lysates of mouse thymus tissue and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1ug/ml of Sheep Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Themis at approximately 73kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Western blot shows lysates of mouse thymus tissue and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1ug/ml of Sheep Mouse/Rat Themis Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Themis at approximately 73kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Related Product Information for anti-Themis antibody
Themis is a 72-74kD member of the Themis family of proteins. It has restricted expression, being found in double negative #2-4 thymocytes, double positive thymocytes, and CD4+ plus CD8+ thymocytes and T cells. The molecule is known to constitute part of the TCR signalosome, along with LAT, Grb2 and PLCg1, and is required for ERK and AP-1 activation. Themis appears to contribute to the process of positive selection. In particular, it is needed to prevent cell death during the selection process. Mouse Themis is 636aa in length. It contains two globular CABIT domains aa1-260, 261-520 plus an NLS aa555-563. There are four isoform variants. One shows a 25aa substitution for aa238-636, a second possesses an alternative start site at Met90 coupled to a 13aa substitution for aa589-636, while third and fourth variants contain 7aa and 17aa substitutions for aa589-636. Over aa122-237, mouse Themis shares 96% and 87% aa identity with rat and human Themis.
Product Categories/Family for anti-Themis antibody
Similar Products
Product Notes
The Themis (Catalog #AAA643337) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Themis can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Themis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Themis, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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