Sheep MIB1 Polyclonal Antibody

MIB1 (E3 Ubiquitin-protein Ligase MIB1, Mind Bomb Homolog 1, DAPK-interacting Protein 1, DIP-1, Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2, MIB1, DIP1, KIAA1323, ZZANK2)

Cat. Num. AAA645447

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MIB1; Polyclonal Antibody; MIB1 (E3 Ubiquitin-protein Ligase MIB1; Mind Bomb Homolog 1; DAPK-interacting Protein 1; DIP-1; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2; DIP1; KIAA1323; ZZANK2); Anti -MIB1 (E3 Ubiquitin-protein Ligase MIB1; anti-MIB1 antibody

• Host: Sheep
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MIB1. Species Crossreactivity: mouse and rat, recombinant MIB2 (<1%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-MIB1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa365-461 from human MIB1 expressed in E.coli.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human, Mouse, and Rat Mind Bomb 1/MIB1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1ug/ml of Sheep Human/Mouse/Rat Mind Bomb1/MIB1 Affinity purified Polyclonal followed by HRP-conjugated AntiSheep IgG Secondary. A specific band was detected for Mind Bomb 1/MIB1 at approximately 115kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-MIB1 antibody

MIB1 (Mind bomb 1 also DIP1 and ZZANK2) is a 110-115kD E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006aa in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc finger motifs. There is one potential alternative start site at Met84. Over aa365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.

Product Categories/Family for anti-MIB1 antibody

Antibodies; Abs to Ubiquitin Ligases