Rabbit anti-Human RPA2 Polyclonal Antibody | anti-RPA2 antibody

RPA2, phosphorylated (T21) (Replication Protein A2)

Cat. Num. AAA642259

• Gene Names: RPA2; REPA2; RPA32
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RPA2; Polyclonal Antibody; phosphorylated (T21) (Replication Protein A2); Anti -RPA2; anti-RPA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPA2, when phosphorylated at T21. Species sequence homology: mouse (83%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, sodium azide, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Applicable Applications for anti-RPA2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to human RPA2 T21 (P15927).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human Phospho­RPA2 (T21) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line untreated (­ ) or treated (+) with 1 μM Camptothecin (CPT) for 1 hour. PVDF Membrane was probed with 0.5ug/ml of Human Phospho­RPA2 (T21) Antigen Affinity­ purified Polyclonal Antibody followed by HRP­conjugated Rabbit IgG Secondary Antibody. A specific band was detected for Phospho­RPA2 (T21) at approximately 40kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-RPA2 antibody

RPA2 is a 32kD DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70kD RPA1 and 14kD RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270aa in length. It contains a Gly/Ser-rich N-terminus (aa1-33), a DNA-binding domain (aa43-171) and a protein-interaction C-terminus (aa187-270). Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N-terminal extensions: one shows an 88aa insertion after Ser4, another shows a 12aa substitution for aa1-4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa93-98, and a potential truncation after Gln175. Over aa141-270, human RPA2 shares 83% aa identity with mouse RPA2.

Product Categories/Family for anti-RPA2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47496643
• NCBI GeneID: 6118
• UniProt Accession #: P15927; Q52II0; Q5TEI9; Q5TEJ5
• Molecular Weight: 29,247 Da
• NCBI Official Full Name: RPA2
• NCBI Official Synonym Full Names: replication protein A2, 32kDa
• NCBI Official Symbol: RPA2
• NCBI Official Synonym Symbols: REPA2; RPA32
• NCBI Protein Information: replication protein A 32 kDa subunit; RP-A p32; RP-A p34; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit
• UniProt Protein Name: Replication protein A 32 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA2
• UniProt Synonym Gene Names: REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34
• UniProt Entry Name: RFA2_HUMAN

Uniprot Description

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; DNA replication factor A complex; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; damaged DNA binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding

Biological Process: G1 DNA damage checkpoint; mismatch repair; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle