Rabbit LAPTM4B Polyclonal Antibody | anti-LAPTM4B antibody

LAPTM4B Antibody

Cat. Num. AAA9603644

• Gene Names: LAPTM4B; LC27; LAPTM4beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: LAPTM4B; Polyclonal Antibody; LAPTM4B Antibody; LAPTM4beta; LC27; Lysosomal associated protein transmembrane 4 beta; Lysosomal associated transmembrane protein 4 beta; Lysosomal associated transmembrane protein 4B; Lysosomal protein transmembrane 4 beta; anti-LAPTM4B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LAPTM4B antibody detects endogenous levels of LAPTM4B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-LAPTM4B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human LAPTM4B.
• Subcellular Location: Endomembrane System; Multipass Membrane Protein.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of rat lymph tissue, using LAPTM4B antibody.)

Immunohistochemistry (IHC)

(MBS9603644 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-LAPTM4B antibody

Function: Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4, 5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).
Subunit Structure: Homooligomer; upon reaching the lysosomes (PubMed:21224396). Interacts with MCOLN1 (PubMed:21224396). Interacts with NEDD4; may play a role in the lysosomal sorting of LAPTM4B; enhances HGS association with NEDD4; mediates inhibition of EGFR degradation (PubMed:22096579, PubMed:25588945). Interacts with PIP5K1C; promotes SNX5 association with LAPTM4B; kinase activity of PIP5K1C is required; interaction is regulated by phosphatidylinositol 4, 5-bisphosphate generated by PIP5K1C (PubMed:25588945). Interacts with HGS; promotes HGS ubiquitination (PubMed:25588945). Interacts with SNX5 (PubMed:25588945). Interacts with SLC3A2 AND SLC7A5; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567). Interacts with LRRC32; decreases TGFB1 production in regulatory T cells (PubMed:26126825). Interacts with BECN1; competes with EGFR for LAPTM4B binding; regulates EGFR activity (PubMed:28479384). Interacts with EGFR; positively correlates with EGFR activation (PubMed:28479384).
Post-translational Modifications: Undergoes proteolytic cleavage following delivery to the lysosomes. Ubiquitinated by NEDD4.
Similarity: Belongs to the LAPTM4/LAPTM5 transporter family.

NCBI and Uniprot Product Information

• NCBI GI #: 30409982
• NCBI GeneID: 55353
• NCBI Accession #: NP_060877.3
• NCBI GenBank Nucleotide #: NM_018407.4
• UniProt Accession #: Q86VI4; Q3ZCV5; Q7L909; Q86VH8; Q9H060
• Molecular Weight: Observed: 24 kDa; Predicted: 42 kDa
• NCBI Official Full Name: lysosomal-associated transmembrane protein 4B
• NCBI Official Synonym Full Names: lysosomal protein transmembrane 4 beta
• NCBI Official Symbol: LAPTM4B
• NCBI Official Synonym Symbols: LC27; LAPTM4beta
• NCBI Protein Information: lysosomal-associated transmembrane protein 4B
• UniProt Protein Name: Lysosomal-associated transmembrane protein 4B
• Protein Family: Lysosomal-associated transmembrane protein
• UniProt Gene Name: LAPTM4B

Uniprot Description

Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).