Western Blot (WB)
(Detection of Human Tensin 4/CTEN by Western Blot.|Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Mouse Human Tensin 4/CTEN Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for Tensin 4/CTEN at approximately 90kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Mouse anti-Human TNS4 Monoclonal Antibody | anti-TNS4 antibody
TNS4 (Tensin 4, CTEN, Tensin-4, C-terminal Tensin-like Protein, PP14434)
Gene Names
TNS4; CTEN; PP14434
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A/G affinity chromatography.
Synonyms
TNS4; Monoclonal Antibody; TNS4 (Tensin 4; CTEN; Tensin-4; C-terminal Tensin-like Protein; PP14434); Anti -TNS4 (Tensin 4; anti-TNS4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
12K185
Specificity
Recognizes human Tensin 4.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Applicable Applications for anti-TNS4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 8-25ug/ml
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 8-25ug/ml
Immunogen
Recombinant corresponding to aa2-107 from human Tensin 4 expressed in E.coli.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human Tensin 4/CTEN by Western Blot.|Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Mouse Human Tensin 4/CTEN Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for Tensin 4/CTEN at approximately 90kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Human Tensin 4/CTEN by Western Blot.|Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Mouse Human Tensin 4/CTEN Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for Tensin 4/CTEN at approximately 90kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Immunohistochemistry (IHC)
(Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Human Tensin 4/ CTEN Monoclonal Antibody at 25ug/ml overnight at 4°C. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.)
Immunohistochemistry (IHC)
(Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Human Tensin 4/ CTEN Monoclonal Antibody at 25ug/ml overnight at 4°C. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.)
Related Product Information for anti-TNS4 antibody
CTEN, also known as Tensin 4, is a 90kD cytoplasmic protein that is associated with focal adhesions in epithelial cells and is upregulated in several carcinomas. It promotes tumorigenesis by disrupting the attachment of focal adhesions to the cytoskeleton, promoting epithelial-mesenchymal transition, and binding nuclear beta-Catenin. CTEN is cleaved by Caspase-3 at aa570-571 during apoptosis. It contains a serine-rich region aa168-324, one SH2 domain aa449-556, and a phosphatase domain aa548-714. Within aa1-107, human CTEN shares 62% and 59% aa sequence identity with mouse and rat CTEN.
Product Categories/Family for anti-TNS4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,764 Da
NCBI Official Full Name
TNS4 protein, partial
NCBI Official Synonym Full Names
tensin 4
NCBI Official Symbol
TNS4
NCBI Official Synonym Symbols
CTEN; PP14434
NCBI Protein Information
tensin-4; C terminal tensin like; C-terminal tensin-like protein
UniProt Protein Name
Tensin-4
Protein Family
UniProt Gene Name
TNS4
UniProt Synonym Gene Names
CTEN
UniProt Entry Name
TENS4_HUMAN
Uniprot Description
TNS4: May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. May promote apoptosis, via its cleavage by caspase-3.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: cytoskeleton; focal adhesion; cytoplasm
Molecular Function: protein binding; actin binding
Biological Process: protein localization; apoptosis
Research Articles on TNS4
Similar Products
Product Notes
The TNS4 tns4 (Catalog #AAA6005076) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNS4 (Tensin 4, CTEN, Tensin-4, C-terminal Tensin-like Protein, PP14434) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNS4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml. Researchers should empirically determine the suitability of the TNS4 tns4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNS4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
