Western Blot (WB)
(Detection of Human Wnt-8b by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1ug/ml of Goat Human/Mouse Wnt-8b Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Goat IgG Secondary Antibody. A specific band was detected for Wnt-8b at approximately 54ka. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Goat anti-Human, Mouse Wnt8b Polyclonal Antibody | anti-WNT8B antibody
Wnt8b (Protein Wnt-8b)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Western Blot (WB)
(Detection of Human Wnt-8b by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1ug/ml of Goat Human/Mouse Wnt-8b Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Goat IgG Secondary Antibody. A specific band was detected for Wnt-8b at approximately 54ka. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Western Blot (WB)
(Detection of Human Wnt-8b by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1ug/ml of Goat Human/Mouse Wnt-8b Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Goat IgG Secondary Antibody. A specific band was detected for Wnt-8b at approximately 54ka. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT8B: Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: frizzled binding
Biological Process: neuron differentiation; nervous system development; cell fate commitment; Wnt receptor signaling pathway; response to retinoic acid; gastrulation; determination of dorsal identity; signal transduction; response to estradiol stimulus
Research Articles on WNT8B
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Product Notes
The WNT8B wnt8b (Catalog #AAA6009730) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Wnt8b (Protein Wnt-8b) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt8b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the WNT8B wnt8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt8b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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