Typical Testing Data/Standard Curve (for reference only)
Rat Interleukin 18 Binding Protein (IL18BP) ELISA Kit | IL18BP elisa kit
Rat Interleukin 18 Binding Protein (IL18BP) ELISA Kit
Gene Names
IL18BP; IL18BPa
Reactivity
Rat
Synonyms
Interleukin 18 Binding Protein (IL18BP); Rat Interleukin 18 Binding Protein (IL18BP) ELISA Kit; IL18BPa; MC51L-53L-54L Homolog Gene Product; Tadekinig-alfa; IL18BP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 18 Binding Protein (IL18BP).
No significant cross-reactivity or interference between Interleukin 18 Binding Protein (IL18BP) and analogues was observed.
No significant cross-reactivity or interference between Interleukin 18 Binding Protein (IL18BP) and analogues was observed.
Sequence Length
163
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.251ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL18BP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Binding Protein (IL18BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 Binding Protein (IL18BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 Binding Protein (IL18BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 Binding Protein (IL18BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for IL18BP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,478 Da
NCBI Official Full Name
Interleukin 18 binding protein
NCBI Official Synonym Full Names
interleukin 18 binding protein
NCBI Official Symbol
IL18BP
NCBI Official Synonym Symbols
IL18BPa
NCBI Protein Information
interleukin-18-binding protein
UniProt Protein Name
Interleukin-18-binding protein
Protein Family
UniProt Gene Name
IL18BP
UniProt Synonym Gene Names
IL-18BP
UniProt Entry Name
I18BP_HUMAN
NCBI Description
The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
IL18BP: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: extracellular space; extracellular region
Molecular Function: receptor antagonist activity; interleukin-18 binding
Biological Process: T-helper 1 type immune response; response to lipopolysaccharide
Research Articles on IL18BP
Similar Products
Product Notes
The Rat IL18BP il18bp (Catalog #AAA2022281) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2022281 ELISA Kit recognizes Rat IL18BP. It is sometimes possible for the material contained within the vial of "Interleukin 18 Binding Protein (IL18BP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
