Western Blot (WB)
(Detection of Human MIG2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5ug/ml of Mouse Human MIG2 Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for MIG2 at approximately 78kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Mouse anti-Human KIND2 Monoclonal Antibody | anti-FERMT2 antibody
KIND2 (Fermitin Family Homolog 2, Kindlin-2, Mitogen-inducible Gene 2 Protein, MIG-2, Pleckstrin Homology Domain-containing Family C Member 1, PH Domain-containing Family C Member 1, FERMT2, MIG2, PLEKHC1)
Gene Names
FERMT2; MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A/G affinity chromatography.
Synonyms
KIND2; Monoclonal Antibody; KIND2 (Fermitin Family Homolog 2; Kindlin-2; Mitogen-inducible Gene 2 Protein; MIG-2; Pleckstrin Homology Domain-containing Family C Member 1; PH Domain-containing Family C Member 1; FERMT2; MIG2; PLEKHC1); Anti -KIND2 (Fermitin Family Homolog 2; anti-FERMT2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
12K122
Specificity
Recognizes human MIG2. Does not react with recombinant human MIG, rhFERMT1 aa514-677, rhMIG2 aa514-680, or rhFERMT3 aa498-667.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Applicable Applications for anti-FERMT2 antibody
Western Blot (WB)
Application Notes
Suitable for use Western Blot.
Dilution: Western Blot: 0.5ug/ml
Dilution: Western Blot: 0.5ug/ml
Immunogen
Recombinant corresponding to aa321-444 from human MIG2 expressed in E.coli.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human MIG2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5ug/ml of Mouse Human MIG2 Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for MIG2 at approximately 78kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Human MIG2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5ug/ml of Mouse Human MIG2 Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for MIG2 at approximately 78kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Related Product Information for anti-FERMT2 antibody
MIG2 also known as FERMT2 or Kindlin-2, is an ~80kD, 680aa, ubiquitously expressed member of the Fermitin/Kindlin family of cytoplasmic adaptor proteins. MIG2 is essential for B1 and B3 integrin activity due to its interactions with regulatory molecules such as talin and ILK. MIG2 contains two FERM domains aa85-326 and aa524-573 and one pleckstrin homology domain aa373-478. Within the region used as an immunogen, human MIG2 shares 98% aa identity with mouse and rat MIG2. Two isoforms 687aa and 633aa have a 7aa insert after 534aa. The shorter isoform also has a 2aa substitution for the C-terminal 57aa of the 680aa form.
Product Categories/Family for anti-FERMT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
77,861 Da
NCBI Official Full Name
KINDLIN2 isoform 2
NCBI Official Synonym Full Names
fermitin family member 2
NCBI Official Symbol
FERMT2
NCBI Official Synonym Symbols
MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
NCBI Protein Information
fermitin family homolog 2; kindlin 2; kindlin-2; mitogen inducible gene 2 protein; mitogen-inducible gene 2 protein; PH domain-containing family C member 1; pleckstrin homology domain-containing family C member 1; pleckstrin homology domain containing, family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1
UniProt Protein Name
Fermitin family homolog 2
Protein Family
UniProt Gene Name
FERMT2
UniProt Synonym Gene Names
KIND2; MIG2; PLEKHC1; MIG-2
UniProt Entry Name
FERM2_HUMAN
Uniprot Description
Kindlin-2: Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Belongs to the kindlin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q22.1
Cellular Component: nucleoplasm; filamentous actin; I band; extrinsic to internal side of plasma membrane; focal adhesion; cell surface; cytoplasm; stress fiber; cell cortex; nucleus; cytosol
Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding
Biological Process: integrin-mediated signaling pathway; focal adhesion formation; integrin activation; regulation of cell shape; Wnt receptor signaling pathway; transforming growth factor beta receptor signaling pathway; cell-matrix adhesion
Research Articles on FERMT2
Similar Products
Product Notes
The FERMT2 fermt2 (Catalog #AAA6003961) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KIND2 (Fermitin Family Homolog 2, Kindlin-2, Mitogen-inducible Gene 2 Protein, MIG-2, Pleckstrin Homology Domain-containing Family C Member 1, PH Domain-containing Family C Member 1, FERMT2, MIG2, PLEKHC1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIND2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use Western Blot. Dilution: Western Blot: 0.5ug/ml. Researchers should empirically determine the suitability of the FERMT2 fermt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIND2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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