Sheep anti-Human TEX19 Polyclonal Antibody | anti-TEX19 antibody

TEX19 (Testis-Expressed Sequence 19 Protein)

Cat. Num. AAA649261

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TEX19; Polyclonal Antibody; TEX19 (Testis-Expressed Sequence 19 Protein); Anti -TEX19 (Testis-Expressed Sequence 19 Protein); anti-TEX19 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TEX19. Species sequence homology: Recombinant human TEX11 (2%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Applicable Applications for anti-TEX19 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 0.5ug/ml; Immunocytochemistry: 10ug/ml
• Immunogen: Recombinant corresponding to aa1-164 from human TEX19 expressed in E. Coli (Q8NA77).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human TEX19 by Western Blot. Western blot shows lysates of NTera­2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5ug/ml of Human TEX19 Antigen Affinity­purified Polyclonal Antibody followed by HRP­conjugated Sheep IgG Secondary Antibody. A specific band was detected for TEX19 at approximately 18kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)

Related Product Information for anti-TEX19 antibody

TEX19 is an 18kD (predicted) protein with restricted cell expression. It is expressed in spermatogonia as well as pluripotent stem cells. TEX19 has been found in both nucleus and cytoplasm. Functionally, TEX19 is proposed to guard against inappropriately activated endogenous retroviruses during meiosis, and thus prevent deleterious mutations. Human TEX19 is 164aa in length. There is no identifiable structural module within the protein. There are, however, two sequences that are conserved in TEX19 from multiple species. These are an MCP domain between aa1-58, and a VPTEL domain that spans aa120-157. Over aa1-164, human TEX19 shares only 53aa identity with a highly divergent and substantially larger 42kD mouse TEX19.1 protein.

Product Categories/Family for anti-TEX19 antibody

Antibodies; Abs to Spermatogenesis; Testis Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 46409542
• NCBI GeneID: 400629
• NCBI Accession #: NP_997342.1
• NCBI GenBank Nucleotide #: NM_207459.3
• UniProt Accession #: Q8NA77
• Molecular Weight: 18,469 Da
• NCBI Official Full Name: testis-expressed sequence 19 protein
• NCBI Official Synonym Full Names: testis expressed 19
• NCBI Official Symbol: TEX19
• NCBI Protein Information: testis-expressed sequence 19 protein
• UniProt Protein Name: Testis-expressed sequence 19 protein
• Protein Family: Testis-expressed sequence 19 protein
• UniProt Gene Name: TEX19
• UniProt Entry Name: TEX19_HUMAN

Uniprot Description

Subcellular location: Nucleus

By similarity.

Tissue specificity: Expressed in testis. Expressed in undifferentiated embryonic stem cells. Ref.4 Ref.5

Induction: Down-regulated by gonadotropin suppression sufficient to cause marked suppression of spermatogenesis and additionally progestogen treatment. Ref.5