Rabbit Themis Polyclonal Antibody | anti-THEMIS antibody

Themis (N-terminal region) Antibody

Cat. Num. AAA474623

• Gene Names: THEMIS; GASP; SPOT; TSEPA; C6orf190; C6orf207
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: Themis; Polyclonal Antibody; Themis (N-terminal region) Antibody; Themis (N-terminal region); anti-THEMIS antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using themis (N-terminal region) peptide (without carrier). The antibody detects a 72kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-THEMIS antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (TX3885) (lanes 4 & 8).)

Related Product Information for anti-THEMIS antibody

During positive selection, thymocytes transition through a stage during which T cell antigen receptor (TCR) signaling controls CD4-versus-CD8 lineage selection and maturation. Thymocyte-expressed molecule involved in selection (themis) is critically required for this thymocyte selection. Themis has been identified as several molecules: Grb2-associating protein (GASP), signaling phosphoprotein specific for T cells (SPoT), TSEPA, and thylex. Themis is a cytosolic protein with no known functional motifs. Its expression is observed primarily in T cells, especially immature CD4/CD8 double positive thymocytes. Themis-deficient thymocytes have defective positive selection, and fewer numbers of mature thymocytes. These themis-induced effects may be due to defective TCR signaling since themis constitutively associates with the TCR adaptor Grb2, and themis is phosphorylated quickly after TCR stimulation. In addition, themis is required for optimal TCR-driven calcium mobilization and activation of the MAP kinase Erk. Thus, themis may be an important adaptor protein in TCR signaling.

References

Fu, G., et al. (2009) Nat Immunol. 10(8):848.
Johnson, A.L., et al. (2009) Nat Immunol. 10(8):831.
Kakugawa, K., et al. (2009) Mol Cell Biol. 29(18):5128.
Lesourne, R., et al. (2009) Nat Immunol. 10(8):840.
Patrick M.S., et al. (2009) Proc Natl Acad Sci U S A. 106(38):16345.

NCBI and Uniprot Product Information

• NCBI GI #: 150421530
• NCBI GeneID: 387357
• UniProt Accession #: Q8N1K5; Q5T3C4; Q5T3C5; Q6MZT7; A1L4F0; A8K7N1; B3KT31; B3KW32; B3KY07; F5H1J9
• Molecular Weight: 77,515 Da
• NCBI Official Full Name: Protein THEMIS
• NCBI Official Synonym Full Names: thymocyte selection associated
• NCBI Official Symbol: THEMIS
• NCBI Official Synonym Symbols: GASP; SPOT; TSEPA; C6orf190; C6orf207
• NCBI Protein Information: protein THEMIS
• UniProt Protein Name: Protein THEMIS
• Protein Family: Protein
• UniProt Gene Name: THEMIS
• UniProt Synonym Gene Names: C6orf190; C6orf207
• UniProt Entry Name: THMS1_HUMAN

NCBI Description

This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

THEMIS: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. Interacts with PLCG1, ITK and GRB2. Belongs to the themis family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytoplasm; intercellular junction; nucleus; signalosome

Molecular Function: protein binding

Biological Process: adaptive immune response; negative T cell selection; positive T cell selection; T cell receptor signaling pathway