SDS-Page
(Recombinant SP140L (401-580) protein gel. SP140L (401-580) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 26.3 kDa Purity: > 90%)
SP140L recombinant protein
Recombinant SP140L (401-580) protein
Purity
The purity ofThe protein is >90% by SDS-PAGE.
Synonyms
SP140L; Recombinant SP140L (401-580) protein; SP140L recombinant protein
Host
E Coli
Purity/Purification
The purity ofThe protein is >90% by SDS-PAGE.
Form/Format
Recombinant SP140L (401-580) protein is supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant SP140L (401-580) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SP140L (401-580) protein gel. SP140L (401-580) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 26.3 kDa Purity: > 90%)
SDS-Page
(Recombinant SP140L (401-580) protein gel. SP140L (401-580) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 26.3 kDa Purity: > 90%)
Testing Data
(HTRF Assay for Recombinant SP140L (401-580) protein activity. 3 uM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant SP140L (401-580) protein activity. 3 uM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant SP140L (401-580) protein activity. 3 uM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant SP140L (401-580) protein activity. 3 uM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for SP140L recombinant protein
Short Description: The peptide corresponding to amino acids 401-580 that contains the bromodomain sequences of SP140L (accession number NP_612411.4) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 27.4 kDa. SP140 nuclear body protein-like (SP140L) is a protein with significant sequence similarity to SP140, a component of the nuclear body that may be involved in trafficking between the nucleus and the cytoplasm. The protein is expressed as four isoforms produced by alternative splicing, SP140L contains one HSR domain, one PHD-type zinc finger, a bromodomain and one SAND domain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. Recombinant SP140L (401-580) can be used in binding assays and inhibitor screening.
Background: SP140 nuclear body protein-like (SP140L) is a protein with significant sequence similarity to SP140, a component of the nuclear body that may be involved in trafficking between the nucleus and the cytoplasm. The protein is expressed as four isoforms produced by alternative splicing, SP140L contains one HSR domain, one PHD-type zinc finger, a bromodomain and one SAND domain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Background: SP140 nuclear body protein-like (SP140L) is a protein with significant sequence similarity to SP140, a component of the nuclear body that may be involved in trafficking between the nucleus and the cytoplasm. The protein is expressed as four isoforms produced by alternative splicing, SP140L contains one HSR domain, one PHD-type zinc finger, a bromodomain and one SAND domain. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Product Categories/Family for SP140L recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,005 Da
NCBI Official Full Name
nuclear body protein SP140-like protein
NCBI Official Synonym Full Names
SP140 nuclear body protein-like
NCBI Official Symbol
SP140L
NCBI Protein Information
nuclear body protein SP140-like protein; SP140L-1 protein; SP140L-2 protein; SP140L-3 protein
UniProt Protein Name
Nuclear body protein SP140-like protein
Protein Family
UniProt Gene Name
SP140L
UniProt Entry Name
SP14L_HUMAN
Uniprot Description
SP140L: 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: nucleus
Molecular Function: DNA binding; zinc ion binding; chromatin binding
Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent
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Product Notes
The SP140L sp140l (Catalog #AAA388151) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SP140L, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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