SDS-Page
(Recombinant KAT2B / PCAF (715-829) protein gel. PCAF (715-829) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
KAT2B/PCAF recombinant protein
Recombinant KAT2B/PCAF (715-829) protein
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
KAT2B/PCAF; Recombinant KAT2B/PCAF (715-829) protein; K (lysine) acetyltransferase 2B (KAT2B); P300/CBP-associated factor; P/CAF; KAT2B/PCAF recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant KAT2B/PCAF (715-829) protein expressed in E Coli and supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant KAT2B/PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KAT2B / PCAF (715-829) protein gel. PCAF (715-829) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant KAT2B / PCAF (715-829) protein gel. PCAF (715-829) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(HTRF Assay for Recombinant KAT2B / PCAF (715-829) protein activity. 3 uM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with KAT2B / PCAF(715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2B / PCAF (715-829) protein activity. 3 uM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with KAT2B / PCAF(715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2B / PCAF (715-829) protein activity. 3 uM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with KAT2B / PCAF (715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2B / PCAF (715-829) protein activity. 3 uM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with KAT2B / PCAF (715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature, Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for KAT2B/PCAF recombinant protein
Short Description: The peptide corresponding to amino acids 715-829 that contains the bromodomain sequences of PCAF (accession number NM_003884.4) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 19.8 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K36, H4K8, H4K16, H4K20 and TATK50. P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT's transactivating activity and may help induce chromatin remodeling of proviral genes. Recombinant PCAF (715-829) can be used in binding assays and inhibitor screening.
Background: P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. KAT2B/PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. KAT2B/PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. KAT2B/PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, KAT2B/PCAF is recruited by the viral protein TAT to regulate TAT's transactivating activity and may help induce chromatin remodeling of proviral genes.
Background: P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. KAT2B/PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. KAT2B/PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. KAT2B/PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, KAT2B/PCAF is recruited by the viral protein TAT to regulate TAT's transactivating activity and may help induce chromatin remodeling of proviral genes.
Product Categories/Family for KAT2B/PCAF recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The KAT2B/PCAF (Catalog #AAA388090) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT2B/PCAF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
