SDS-Page
(Recombinant BAZ1B (1340-1457) protein gel. BAZ1B (1340-1457) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.8 kDa Purity: > 75%)
BAZ1B recombinant protein
Recombinant BAZ1B (1340-1457) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BAZ1B (1340-1457) protein gel. BAZ1B (1340-1457) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.8 kDa Purity: > 75%)
SDS-Page
(Recombinant BAZ1B (1340-1457) protein gel. BAZ1B (1340-1457) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.8 kDa Purity: > 75%)
Testing Data
(HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of BAZ1B (1340- 1457) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. )
Testing Data
(HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of BAZ1B (1340- 1457) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. )
Testing Data
(HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of BAZ1B (1340- 1457) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. )
Testing Data
(HTRF Assay for Recombinant BAZ1B (1340-1457) protein activity. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of BAZ1B (1340- 1457) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. )
Background: Bromodomain adjacent to zinc finger domain, 1B (BAZ1B) is a member of the bromodomain protein family. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BAZ1B is an atypical tyrosine protein kinase that plays a central role in chromatin remodeling and acts as a transcriptional regulator. It is an essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes and has a role in RNA polymerase I and RNA polymerase III transcription. In the complex, BAZ1B mediates the recruitment of the WICH complex to replication foci during DNA replication. BAZ1B and the WICH complex are also involved in DNA damage response through phosphorylation of Tyr142 of histone H2AX (H2AXY142ph), a mark that distinguishes between apoptotic and repair responses to genotoxic stress. BAZ1B is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, BAZ1B plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Deletion of BAZ1B leads to Williams-Beuren syndrome.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. [provided by RefSeq, Jul 2008]
Uniprot Description
WSTF: plays a central role in chromatin remodeling and acts as a transcription regulator. Apparently possesses tyrosine-protein kinase activity, but bears no sequence resemblance classical tyrosine kinase proteins. Involved in DNA damage response by phosphorylating Y142 of histone H2AX. H2AXpY142 plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at Y142, and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. Two alternatively-spliced human isoforms have been reported.
Protein type: Protein kinase, Ser/Thr (non-receptor); DNA replication; Kinase, protein; EC 2.7.10.2; Nuclear receptor co-regulator; ATYPICAL group; BAZ family
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: centric heterochromatin; nuclear replication fork; condensed chromosome
Molecular Function: protein binding; zinc ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; histone kinase activity; chromatin binding; ATP binding
Biological Process: chromatin assembly or disassembly; heart morphogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone phosphorylation; double-strand break repair; response to DNA damage stimulus
Research Articles on BAZ1B
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Product Notes
The BAZ1B baz1b (Catalog #AAA388158) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BAZ1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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