SDS-Page
(Recombinant TRIM24 (862-980) protein gel.TRIM24 (862-980) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.8 kDa Purity: > 95% )
TRIM24 recombinant protein
Recombinant TRIM24 (862-980) protein
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
TRIM24; Recombinant TRIM24 (862-980) protein; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA; TRIM24 recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant TRIM24 (862-980) protein expressed in E Coli and supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant TRIM24 (862-980) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TRIM24 (862-980) protein gel.TRIM24 (862-980) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.8 kDa Purity: > 95% )
SDS-Page
(Recombinant TRIM24 (862-980) protein gel.TRIM24 (862-980) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 18.8 kDa Purity: > 95% )
Testing Data
(HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
Testing Data
(HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
Testing Data
(HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
Testing Data
(HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
Related Product Information for TRIM24 recombinant protein
Short Description: The peptide corresponding to amino acids 862-980 that contains the bromodomain sequences of TRIM24 (accession number NM_003852.3) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 18.8 kDa. It shows binding specificity for acetylated H3K9, H3K14 and H3K16. Tripartite motif-containing 24 (TRIM24) protein, also known as TIF1a, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM24 interacts with chromatin through recognition of specific histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at lysine 4 (H3K4me0) and acetylated at lysine 23 (H3K23ac). TRIM24 is an E3 ubiquitin-protein ligase that promotes proteosomal degradation of p53/TP53 and, in conjunction with TRIM33, mediates cell proliferation and apoptosis. TRIM24 also modulates transcriptional activation by retinoic acid (RA) receptors, including RARA, and has been shown to regulate RA-dependent proliferation of hepatocytes. TRIM24 functions as a transcriptional coactivator that interacts with the AF2 region of numerous nuclear receptors, including estrogen, RA and vitamin D3 receptors, and coactivators to modulate the transcription of target genes. It has been shown to be involved in upregulating ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Recombinant TRIM24 (862-980) can be used in binding assays and inhibitor screening.
Background: Tripartite motif-containing 24 (TRIM24) protein, also known as TIF1a, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM24 interacts with chromatin through recognition of specific histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at lysine 4 (H3K4me0) and acetylated at lysine 23 (H3K23ac). TRIM24 is an E3 ubiquitin-protein ligase that promotes proteosomal degradation of p53/TP53 and, in conjunction with TRIM33, mediates cell proliferation and apoptosis. TRIM24 also modulates transcriptional activation by retinoic acid (RA) receptors, including RARA, and has been shown to regulate RA-dependent proliferation of hepatocytes. TRIM24 functions as a transcriptional coactivator that interacts with the AF2 region of numerous nuclear receptors, including estrogen, RA and vitamin D3 receptors, and coactivators to modulate the transcription of target genes. It has been shown to be involved in upregulating ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1.
Background: Tripartite motif-containing 24 (TRIM24) protein, also known as TIF1a, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM24 interacts with chromatin through recognition of specific histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at lysine 4 (H3K4me0) and acetylated at lysine 23 (H3K23ac). TRIM24 is an E3 ubiquitin-protein ligase that promotes proteosomal degradation of p53/TP53 and, in conjunction with TRIM33, mediates cell proliferation and apoptosis. TRIM24 also modulates transcriptional activation by retinoic acid (RA) receptors, including RARA, and has been shown to regulate RA-dependent proliferation of hepatocytes. TRIM24 functions as a transcriptional coactivator that interacts with the AF2 region of numerous nuclear receptors, including estrogen, RA and vitamin D3 receptors, and coactivators to modulate the transcription of target genes. It has been shown to be involved in upregulating ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1.
Product Categories/Family for TRIM24 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 18.8kDa
Protein Family
Similar Products
Product Notes
The TRIM24 (Catalog #AAA388088) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TRIM24, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
