SDS-Page
(Recombinant KAT2A (GCN5) (726-837) protein gel. GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW:19.4 kDa Purity: > 90%)
KAT2A recombinant protein
Recombinant KAT2A (GCN5)(726-837) protein
Synonyms
KAT2A; Recombinant KAT2A (GCN5)(726-837) protein; hGCN5; GCN5L2; PCAF-b; KAT2A recombinant protein
Host
E Coli
Form/Format
Recombinant KAT2A (GCN5)(726-837) protein is supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant KAT2A (GCN5)(726-837) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KAT2A (GCN5) (726-837) protein gel. GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW:19.4 kDa Purity: > 90%)
SDS-Page
(Recombinant KAT2A (GCN5) (726-837) protein gel. GCN5 (726-837) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW:19.4 kDa Purity: > 90%)
Testing Data
(HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 uM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 uM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 uM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant KAT2A (GCN5) (726-837) activity. 3 uM histone peptide H4K5/8/12/16(4ac) was incubated with KAT2A (GCN5) (726-837) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for KAT2A recombinant protein
Short Description: The peptide corresponding to amino acids 726-837 that contains the bromodomain sequences of GCN5 (accession number NM_021078.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H2AK5, H3K9, H3K14, H3K9/K14, H4K8/K16, H4K16 and H4K5/8/12/16. GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. GCN5 acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests GCN5 may play a role in the induction of chromatin remodeling of proviral genes. GCN5 also functions as a repressor of NFkB by promoting ubiquitination of the NFkB subunit RelA in a HAT-independent manner. GCN5 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. Recombinant GCN5 (726-837) can be used in binding assays and inhibitor screening.
Background: GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. KAT2A (GCN5) acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests KAT2A (GCN5) may play a role in the induction of chromatin remodeling of proviral genes. KAT2A (GCN5) also functions as a repressor of NFkB by promoting ubiquitination of the NFkB subunit RelA in a HAT-independent manner. KAT2A (GCN5) contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Background: GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. KAT2A (GCN5) acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests KAT2A (GCN5) may play a role in the induction of chromatin remodeling of proviral genes. KAT2A (GCN5) also functions as a repressor of NFkB by promoting ubiquitination of the NFkB subunit RelA in a HAT-independent manner. KAT2A (GCN5) contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Product Categories/Family for KAT2A recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 19.4kDa
Protein Family
Similar Products
Product Notes
The KAT2A (Catalog #AAA388091) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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