SDS-Page
(Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel.BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
BRPF1 recombinant protein
Recombinant BRPF1 (629-742), GST-Tag
Purity
The purity of BRPF1 (629-742), GST-Tag is >92% pure by SDS-PAGE.
Synonyms
BRPF1; Recombinant BRPF1 (629-742); GST-Tag; Bromodomain And PHD Finger Containing 1; Peregrin; BR140; BRPF1 recombinant protein
Host
E Coli
Purity/Purification
The purity of BRPF1 (629-742), GST-Tag is >92% pure by SDS-PAGE.
Form/Format
Recombinant BRPF1 (629-742), GST-Tag is suppled in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant BRPF1 (629-742), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel.BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel.BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(HTRF assay for BRPF1 (629-742) protein activity.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.)
Testing Data
(HTRF assay for BRPF1 (629-742) protein activity.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.)
Testing Data
(HTRF assay for BRPF1 (629-742) protein activity.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.)
Testing Data
(HTRF assay for BRPF1 (629-742) protein activity.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.)
Related Product Information for BRPF1 recombinant protein
Short Description: The peptide corresponding to amino acids 629-742 that contains the bromodomain sequences of BRPF1 (accession number NP_004625.2) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 39.4 kDa. BRPF1 (629-742), GST-Tag is >92% pure by SDS-PAGE. Recombinant BRPF1 (629-742), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: Bromodomain and PHD finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.
Background: Bromodomain and PHD finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.
Product Categories/Family for BRPF1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of 39.4kDa
Protein Family
Similar Products
Product Notes
The BRPF1 (Catalog #AAA388251) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRPF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
