BRDT recombinant protein

Recombinant BRDT (257-382), GST-Tag

Cat. Num. AAA388194

• Gene Names: BRDT; CT9; BRD6
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: BRDT; Recombinant BRDT (257-382); GST-Tag; CT9; BRD6; BRDT recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant BRDT (257-382), GST-Tag protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

• Protein Species: Human
• Tag: GST-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant BRDT (257-382), GST-Tag protein gel. BRDT (257-382), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(Recombinant BRDT (257-382) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)

Testing Data

(Recombinant BRDT (257-382) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)

Related Product Information for BRDT recombinant protein

Short Description: The peptide corresponding to amino acids 257-382 that contains the second bromodomain sequences of BRDT (accession number NP_997072.1) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.8 kDa. The recombinant protein is >90% pure by SDS-PAGE.

Background: BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

Product Categories/Family for BRDT recombinant protein

Recombinant Proteins; Bromodomain

NCBI and Uniprot Product Information

• NCBI GI #: 46399200
• NCBI GeneID: 676
• NCBI Accession #: NP_997072.1
• NCBI GenBank Nucleotide #: NM_207189.2
• UniProt Accession #: Q58F21; O14789; Q6P5T1; Q7Z4A6; Q8IWI6; Q05DQ4
• Molecular Weight: 107,954 Da
• NCBI Official Full Name: bromodomain testis-specific protein isoform b
• NCBI Official Synonym Full Names: bromodomain, testis-specific
• NCBI Official Symbol: BRDT
• NCBI Official Synonym Symbols: CT9; BRD6
• NCBI Protein Information: bromodomain testis-specific protein; OTTHUMP00000011809; OTTHUMP00000011810; OTTHUMP00000197577; RING3-like protein; cancer/testis antigen 9
• UniProt Protein Name: Bromodomain testis-specific protein
• Protein Family: Bromodomain testis-specific protein
• UniProt Gene Name: BRDT
• UniProt Synonym Gene Names: CT9

Uniprot Description

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.