ATAD2 recombinant protein

Recombinant ATAD2 (981-1108) protein

Cat. Num. AAA388096

• Gene Names: ATAD2; ANCCA; CT137; PRO2000
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: ATAD2; Recombinant ATAD2 (981-1108) protein; ANCCA; CT137; PRO2000; ATAD2 recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant ATAD2 (981-1108) protein was expressed in E Coli cells and is supplied in 25mM Tris-HCl pH8.0, 500mM NaCl, 20% glycerol.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: Recombinant ATAD2 (981-1108) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant ATAD2 (981-1108) protein gel. ATAD2 (981-1108) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)

Testing Data

(Recombinant ATAD2 (981-1108) HTRF activity assay. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with ATAD2 (981-1108) in reaction buffer including 50 mM HEPES-NaOH pH 7.5 and 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)

Related Product Information for ATAD2 recombinant protein

Short Description: The peptide corresponding to amino acids 981-1108 that contains the bromodomain sequences of ATAD2 (accession number NP_054828.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 21.317 kDa. It shows binding specificity for acetylated H3K14. ATPase family AAA domain-containing 2 (ATAD2) protein belongs to the AAA protein family which includes a diverse set of proteins with the capacity to couple ATPase-derived chemical energy to mechanical energy resulting in remodeling or translocation of the targeted macromolecular substrate. These proteins are involved in a number of cellular processes ranging from protein degradation and DNA replication to membrane fusion and the movement of microtubule motors. ATAD2 is thought to act as a transcriptional coactivator of the nuclear receptor ESR1 that is required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. Furthermore, ATAD2 may also play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. In addition, there is evidence that ATAD2 may be required for histone hyperacetylation. Human ATAD2 consists of two AAA+ domains and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The ATAD2 bromodomain specifically interacts with H4K5ac and may contribute to cancer cell proliferation since it has been associated with poor prognosis in breast and testicular cancer. Recombinant ATAD2 (981-1108) can be used in binding assays and inhibitor screening.

Background: ATPase family AAA domain-containing 2 (ATAD2) protein belongs to the AAA protein family which includes a diverse set of proteins with the capacity to couple ATPase-derived chemical energy to mechanical energy resulting in remodeling or translocation of the targeted macromolecular substrate. These proteins are involved in a number of cellular processes ranging from protein degradation and DNA replication to membrane fusion and the movement of microtubule motors. ATAD2 is thought to act as a transcriptional coactivator of the nuclear receptor ESR1 that is required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. Furthermore, ATAD2 may also play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. In addition, there is evidence that ATAD2 may be required for histone hyperacetylation. Human ATAD2 consists of two AAA+ domains and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The ATAD2 bromodomain specifically interacts with H4K5ac and may contribute to cancer cell proliferation since it has been associated with poor prognosis in breast and testicular cancer.

Product Categories/Family for ATAD2 recombinant protein

Recombinant Proteins; Bromodomain

NCBI and Uniprot Product Information

• NCBI GI #: 24497618
• NCBI GeneID: 29028
• NCBI Accession #: NP_054828.2
• NCBI GenBank Nucleotide #: NM_014109.3
• Molecular Weight: 47,986 Da
• NCBI Official Full Name: ATPase family AAA domain-containing protein 2
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 2
• NCBI Official Symbol: ATAD2
• NCBI Official Synonym Symbols: ANCCA; CT137; PRO2000
• NCBI Protein Information: ATPase family AAA domain-containing protein 2
• Protein Family: ATPase family AAA domain-containing protein

NCBI Description

A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008]