SDS-Page
(Recombinant BRWD1 (1310-1430) protein gel. BRWD1 (1310-1430) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 19 kDa Purity: > 92%)
BRWD1 recombinant protein
Recombinant BRWD1 (1310-1430) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRWD1 (1310-1430) protein gel. BRWD1 (1310-1430) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 19 kDa Purity: > 92%)
SDS-Page
(Recombinant BRWD1 (1310-1430) protein gel. BRWD1 (1310-1430) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 19 kDa Purity: > 92%)
Testing Data
(HTRF assay for BRWD1 (1310-1430) activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF assay for BRWD1 (1310-1430) activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Background: BRWD1 (Bromodomain and WD repeat domain containing 1) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. In mouse, this BRWD1 is a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain. This domain associates with a component of the SWI/SNF chromatin remodeling complex and may function to regulate chromatin remodeling. BRWD1 plays a role in the regulation of cell morphology and cytoskeletal organization and is also required in the control of cell shape.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex.[provided by RefSeq, Jun 2011]
Research Articles on BRWD1
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Product Notes
The BRWD1 (Catalog #AAA388154) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRWD1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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