SDS-Page
(Recombinant BRD2 (344-455), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.3 kDa Purity: >85%)
BRD2 recombinant protein
Recombinant BRD2 (344-455) protein, GST-Tag
Synonyms
BRD2; Recombinant BRD2 (344-455) protein; GST-Tag; Bromodomain Containing 2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E; BRD2 recombinant protein
Host
E Coli
Form/Format
Recombinant BRD2 (344-455), GST-Tag protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRD2 (344-455), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.3 kDa Purity: >85%)
SDS-Page
(Recombinant BRD2 (344-455), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.3 kDa Purity: >85%)
Testing Data
(HTRF assay for BRD2 (344-455), GST-Tag activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD2 (344-455), GST-tag protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for BRD2 (344-455), GST-Tag activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD2 (344-455), GST-tag protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for BRD2 (344-455), GST-Tag activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD2 (344-455), GST-tag protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for BRD2 (344-455), GST-Tag activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD2 (344-455), GST-tag protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for BRD2 recombinant protein
Short Description: Recombinant BRD2 (344-455), GST-Tag protein includes amino acids 344-455 (the second Bromodomain) of human BRD2 protein (accession number NP_005095.1); it was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 40.3 kDa. This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. It shows binding specificity for acetylated H4K5/K8, H4K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16.
Background: Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. It shows binding specificity for acetylated H4K5/K8, H4K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16.
Product Categories/Family for BRD2 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of 40.3kDa
Protein Family
Similar Products
Product Notes
The BRD2 (Catalog #AAA389207) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRD2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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