Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
2006 results for "Goat IgG Isotype Control" - showing 150-200
SDS-Page
(SDS-PAGE Analysis Purified CD79a Mouse Monoclonal Antibody (ZL7-4). Confirmation of Integrity and Purity of Antibody.)
CD79a, Monoclonal Antibody (Cat# AAA4381447)
Full Name
CD79a (B-Cell Marker)
Gene Names
CD79A; IGA; MB-1
Reactivity
Human
Applications
EIA, FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808790)
Full Name
Methylglyoxal Antibody, Clone 9E7
Applications
WB, ICC, IF, IHC, FC
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PTP4A2 using anti-PTP4A2 antibody (MBS177865).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Skeletal Muscle Tissue Lysate,Lane 2: Rat Thymus Tissue Lysate,Lane 3: Mouse Brain Tissue Lysate,Lane 4: Mouse Thymus Tissue Lysate,Lane 5: 22RV1 Whole Cell Lysate,Lane 6: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP4A2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTP4A2 at approximately 19KD. The expected band size for PTP4A2 is at 19KD. )
PTP4A2, Polyclonal Antibody (Cat# AAA177865)
Full Name
Anti-PTP4A2 Antibody
Gene Names
PTP4A2; HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808803)
Full Name
Methylglyoxal Antibody, Clone 9E7: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Recombinant Monoclonal Antibody (rPTPRC/1460).)
CD45/LCA, Antibody (Cat# AAA4381214)
Full Name
CD45/LCA (B-Cell Marker)
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808763)
Full Name
Malondialdehyde Antibody, Clone 6H6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Granulocytes, Monoclonal Antibody (Cat# AAA225668)
Full Name
Mouse Anti Pig Granulocytes
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$395/0.1 mg | $1,630/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (MBS1750687).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human 22RV1 whole cell lysates,Lane 4: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD. )
SCRIBBLE, Polyclonal Antibody (Cat# AAA1750687)
Full Name
Anti-SCRIBBLE Picoband antibody
Gene Names
SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
CD172a, Monoclonal Antibody (Cat# AAA212963)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Caspase3 using anti-Caspase3 antibody (MBS177469).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.lane 1: recombinant human Caspase3 protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase3 at approximately 39KD. The expected band size for Caspase3 is at 39KD. )
Caspase-3, Polyclonal Antibody (Cat# AAA177469)
Full Name
Anti-Caspase-3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
WB, IHC-P
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using VWF. MBS9200676 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
VWF, Monoclonal Antibody (Cat# AAA9200676)
Full Name
VWF Antibody
Gene Names
VWF; VWD; F8VWF
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-Fibrin beta-chain antibody 8E5 HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of 8E5 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fibrin beta-Chain, Monoclonal Recombinant Antibody (Cat# AAA488466)
Full Name
Anti-Fibrin beta-Chain [8E5]
Gene Names
FGB; HEL-S-78p
Reactivity
Human
Applications
EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometric
(Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (B-BI2) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red))
CD3, Monoclonal Antibody (Cat# AAA439308)
Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of beta Amyloid using anti-beta Amyloid antibody (MBS176795). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human beta-Amyloid protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Amyloid antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for beta Amyloid at approximately 29KD. The expected band size for beta Amyloid is at 29KD. )
beta Amyloid, Polyclonal Antibody (Cat# AAA176795)
Full Name
Anti-beta Amyloid Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(All lanes : ACBD5 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.)
ACBD5, Monoclonal Antibody (Cat# AAA435111)
Full Name
ACBD5 Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
CD3E, Monoclonal Antibody (Cat# AAA435170)
Full Name
CD3E (754) Mouse mAb
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CAPN2, Monoclonal Antibody (Cat# AAA9231851)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC-P, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BRD3 monoclonal antibody. )
BRD3, Monoclonal Antibody (Cat# AAA120113)
Full Name
Mouse monoclonal antibody Anti-Human BRD3
Gene Names
BRD3; ORFX; RING3L
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(ATPA was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25203 of 10 ug. Western blot was performed from the immunoprecipitate using M25203 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction. Blocking and dilution buffer and concentration:5% milk/TBST.)
ATP5A, Monoclonal Antibody (Cat# AAA435146)
Full Name
ATP5A Mouse mAb
Gene Names
ATP5PF; F6; CF6; ATP5; ATPM; ATP5A; ATP5J
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Quality Control
(Left: Western blot analysis of immunized recombinant protein, using anti-IRF3 monoclonal antibody.)
IRF3, Monoclonal Antibody (Cat# AAA120017)
Full Name
Mouse monoclonal antibody Anti-Human IRF3
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Testing Data
(Figure A. FITC conjugated mouse anti porcine CD3 and purified mouse IgG2a isotype control detected with RPE conjugated goat anti mouse IgG . Figure B. FITC conjugated mouse anti porcine CD3 and purified mouse anti porcine CD45 detected with RPE conjugated goat anti mouse IgG . All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
CD45, Monoclonal Antibody (Cat# AAA225627)
Full Name
Mouse Anti Pig CD45 Allotypic Variant
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Pig
Applications
FC/FACS, IP, IHC
Purity
Purified IgG prepared by affinity chromatography on Protein A
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808589)
Full Name
Acrolein Antibody, Clone 2H2: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclophilin A using anti- Cyclophilin A antibody (MBS1750616).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cyclophilin A antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin A at approximately 18KD. The expected band size for Cyclophilin A is at 18KD. )
Cyclophilin A, Polyclonal Antibody (Cat# AAA1750616)
Full Name
Anti-Cyclophilin A Picoband Antibody
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(1:2500 dilution of this antibody detected CRTC1 on 40ug of cell lysates)
CRTC1, Monoclonal Antibody (Cat# AAA9400213)
Full Name
CRTC1 Monoclonal Antibody
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human, Porcine, Mouse, Rat
Applications
WB, ICC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
IHC-P
(Staining PLA2G7 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
PLA2G7, Polyclonal Antibody (Cat# AAA9205733)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
IHC-P, FC, WB, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate )
Tomm20, Monoclonal Antibody (Cat# AAA206598)
Full Name
Tomm20 antibody
Gene Names
TOMM20; MAS20; MOM19; TOM20
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using GATA3 Antibody (Center)(green) compared to an isotype control of mouse IgG2b(blue). MBS9200559 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
GATA3, Monoclonal Antibody (Cat# AAA9200559)
Full Name
GATA3 Antibody (Center)
Gene Names
GATA3; HDR; HDRS
Reactivity
Human
Applications
WB, EIA, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EZH2, Polyclonal Antibody (Cat# AAA9202800)
Full Name
EZH2 Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CD90/Thy1 using anti- CD90/Thy1 antibody (MBS1750721).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: rat thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD. )
CD90/Thy1, Polyclonal Antibody (Cat# AAA1750721)
Full Name
Anti-CD90/Thy1 Picoband Antibody
Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (MBS1750760).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD. )
Synaptotagmin 1, Polyclonal Antibody (Cat# AAA1750760)
Full Name
Anti-Synaptotagmin 1 Picoband antibody
Gene Names
SYT1; P65; SYT; SVP65
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
P5CS, Monoclonal Antibody (Cat# AAA435154)
Full Name
P5CS (012) Mouse mAb
Gene Names
ALDH18A1; GSAS; P5CS; PYCS; SPG9; ADCL3; SPG9A; SPG9B; ARCL3A
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of immunized recombinant protein, using anti-CARD8 monoclonal antibody. )
CARD8, Monoclonal Antibody (Cat# AAA120117)
Full Name
Mouse monoclonal antibody Anti-Human CARD8
Gene Names
CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
Reactivity
Human
Applications
WB, IP, FC, ICC
Purity
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
ATP synthase subunit gamma, Monoclonal Antibody (Cat# AAA435251)
Full Name
ATP synthase subunit gamma Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fas antibody R-125224 (Ab00802) Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (Ab00802-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fas, Monoclonal Recombinant Antibody (Cat# AAA488362)
Full Name
Anti-Fas [R-125224]
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody. )
TFAP2A, Monoclonal Antibody (Cat# AAA120100)
Full Name
Mouse monoclonal antibody Anti-Human TFAP2A
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human
Applications
DB, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PNP using anti-PNP antibody (MBS178628).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: rat ovary tissue lysates,Lane 3: mouse liver tissue lysates,Lane 4: human placneta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PNP at approximately 38KD. The expected band size for PNP is at 38KD. )
PNP, Polyclonal Antibody (Cat# AAA178628)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215797)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$375/0.1 mg | $1,535/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200385 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
CAPN1, Monoclonal Antibody (Cat# AAA9200385)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). MBS9212584 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Pbk, Polyclonal Antibody (Cat# AAA9212584)
Full Name
Mouse Pbk Antibody (N-term)
Gene Names
Pbk; TOPK; AW538537; D14Ertd732e; 2810434B10Rik
Reactivity
Human, Mouse
Applications
EIA, IHC-P, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing ZF4 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ak2, Polyclonal Antibody (Cat# AAA9232174)
Full Name
Zebrafish ak2 Antibody (N-term)
Gene Names
ak2; wu:fb34f05; wu:fj80e03
Reactivity
Zebrafish
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
ELISA (EIA)
( Binding curve of anti-ICOSL antibody HK5.3 to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
ICOSL, Monoclonal Recombinant Antibody (Cat# AAA488367)
Full Name
Anti-ICOSL [HK5.3]
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
( Binding curve of anti-B7-H3 antibody MJ18 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
B7-H3, Monoclonal Recombinant Antibody (Cat# AAA488371)
Full Name
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808698)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Western Blot (WB)
(Western blot detection of SMC1A (N-terminus) in HelaNE, Jurkat and A431 cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.)
SMC1A, Monoclonal Antibody (Cat# AAA475071)
Full Name
Anti-SMC1A (N-term) Mouse mAb
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808665)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5E8: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of CD20 using anti-CD20 antibody (MBS176945).CD20 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD20 Antibody (MBS176945) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CD20, Polyclonal Antibody (Cat# AAA176945)
Full Name
Anti-CD20 Antibody
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(All lanes : KTN1 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 156 KDa Observed band size : 156 KDa Blocking/Dilution buffer : 1x TBST.)
KTN1, Monoclonal Antibody (Cat# AAA435112)
Full Name
KTN1 (582) Mouse mAb
Gene Names
KTN1; CG1; KNT; MU-RMS-40.19
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PKC eta using anti-PKC eta antibody (MBS178258). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate Lane 4: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD. )
PKC eta, Polyclonal Antibody (Cat# AAA178258)
Full Name
Anti-PKC eta Antibody
Gene Names
PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
Reactivity
Human
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
G3BP, Monoclonal Antibody (Cat# AAA435094)
Full Name
G3BP Mouse mAb
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of POR using anti-POR antibody (MBS178155).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver Tissue Lysate,Lane 2: Human Placenta Tissue Lysate,Lane 3: HEPG2 Whole Cell Lysate,Lane 4: HEPA Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POR at approximately 85KD. The expected band size for POR is at 77KD. )
POR, Polyclonal Antibody (Cat# AAA178155)
Full Name
Anti-POR Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
