Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Mouse anti-Pig Granulocytes Monoclonal Antibody
Mouse Anti Pig Granulocytes
0.09% Sodium Azide (NaN3)
Pack Size: 0.25 mg
0.09% Sodium Azide (NaN3)
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell monocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse IgG1 isotype control Figure B. FITC conjugated Mouse anti Pig CD172a and Alexa Fluor 647 conjugated Mouse anti Pig granulocytes . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell monocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse IgG1 isotype control detected with FITC conjugated Goat anti Mouse IgG1 Figure B. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse anti Pig granulocytes detected with FITC conjugated Goat anti Mouse IgG1 . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse IgG1 isotype control detected with FITC conjugated Goat anti Mouse IgG1 Figure B. Alexa Fluor 647 conjugated Mouse anti Pig granulocytes and Mouse anti Pig granulocytes detected with FITC conjugated Goat anti Mouse IgG1 . All experiments performed on porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse anti Pig granulocytes . All experiments performed on red cell lysed porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig monocyte/granulocyte and FITC conjugated Mouse anti Pig granulocytes . All experiments performed on red cell lysed porcine blood blocked with 10% porcine serum and gated on live, single cell granulocytes. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Published Customer Image:Mouse Anti Porcine granulocytes antibody, clone 2B2 used to identify mature neutrophils in porcine blood by flow cytometery in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 expressed on both immature and mature porcine neutrophils.Image caption:Gating strategy.Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSChigh 6D10+ 2B2-) and mature neutrophils (CD172a+ SSChigh 6D10+ 2B2+).From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.Delayed development of systemic immunity in preterm pigs as a model for preterm infants.Sci Rep. 2016 Nov 10;6:36816.)
Testing Data
(Published Customer Image:Mouse Anti Porcine granulocytes antibody, clone 2B2 used to identify mature neutrophils in porcine blood by flow cytometery in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 expressed on both immature and mature porcine neutrophils.Image caption:Gating strategy.Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSChigh 6D10+ 2B2-) and mature neutrophils (CD172a+ SSChigh 6D10+ 2B2+).From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.Delayed development of systemic immunity in preterm pigs as a model for preterm infants.Sci Rep. 2016 Nov 10;6:36816.)
Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Perez et al. 2007).
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Product Notes
The Granulocytes (Catalog #AAA225668) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Pig Granulocytes reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Granulocytes can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 1/100-1/200. Researchers should empirically determine the suitability of the Granulocytes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granulocytes, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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