Mouse anti-Human Tomm20 Monoclonal Antibody | anti-Tomm20 antibody

Tomm20 antibody

Cat. Num. AAA206598

• Gene Names: TOMM20; MAS20; MOM19; TOM20
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: By protein-A affinity chromatography
• Synonyms: Tomm20; Monoclonal Antibody; Tomm20 antibody; Mmitochondrial import receptor subunit TOM20 homolog; MAS20; MOM19; TOM20; anti-Tomm20 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: AT1B2
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 145

• Applicable Applications for anti-Tomm20 antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Immunogen: Recombinant human Tomm20 (25-145aa) purified from E. coli
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of Tomm20 in HeLa cells. The cell was stained with MBS206598 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of Tomm20 in HeLa cells. The cell was stained with MBS206598 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). )

Related Product Information for anti-Tomm20 antibody

Mitochondrial import receptor subunit TOMM20 homolog, also known as TOMM20, belongs to the Tom20 family. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. TOMM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Product Categories/Family for anti-Tomm20 antibody

Signal Transduction

References

Ahting U., et al. (1999) J Cell Biol. 147: 959-968.; Brix J., et al. (1999) J Biol Chem. 274: 16522-16530.

NCBI and Uniprot Product Information

• NCBI GI #: 7657257
• NCBI GeneID: 9804
• NCBI Accession #: NP_055580
• NCBI GenBank Nucleotide #: NM_014765.2
• UniProt Accession #: Q15388; Q498B3; Q6IBT4; A8K195
• NCBI Official Full Name: mitochondrial import receptor subunit TOM20 homolog
• NCBI Official Synonym Full Names: translocase of outer mitochondrial membrane 20
• NCBI Official Symbol: TOMM20
• NCBI Official Synonym Symbols: MAS20; MOM19; TOM20
• NCBI Protein Information: mitochondrial import receptor subunit TOM20 homolog
• UniProt Protein Name: Mitochondrial import receptor subunit TOM20 homolog
• Protein Family: TOMM20-like protein
• UniProt Gene Name: TOMM20
• UniProt Synonym Gene Names: KIAA0016

Uniprot Description

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore ().