Mouse anti-Human CARD8 Monoclonal Antibody | anti-CARD8 antibody

Mouse monoclonal antibody Anti-Human CARD8

Cat. Num. AAA120117

• Gene Names: CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Synonyms: CARD8; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human CARD8; Homo sapiens caspase recruitment domain family; member 8 (CARD8); DACAR; NDPP1; TUCAN; CARDINAL; KIAA0955;MGC57162; DKFZp779L0366; anti-CARD8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2108C2a
• Purity/Purification: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Form/Format: Mouse monoclonal anti-human CARD8 antibody in PBS (3.0 mM KCl, 1.5 mM KH₂PO₄, 140 mM NaCl, 8.0 mM Na₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃).

• Applicable Applications for anti-CARD8 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)
• Application Notes: Recommended Condition: ; WB: 0.2-2 ug/ml; IP: 100-500 ug/sample; FC: 0.5-2 ug/sample; ICC: 2-100 ug/ml
• Immunogen: Recombinant
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN₃) as a preservative. There is a potential hazard that NaN₃ reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8°C for up to one year. We recommend storing at -20°C for long-term storage. Avoid repeat freezing and thawing cycles.

Western Blot (WB)

(Western blot analysis of immunized recombinant protein, using anti-CARD8 monoclonal antibody. )

Quality Control

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of human CARD8 by Western blot.Samples: Whole cell lysate (50 ug) from HeLa cells. [Lot No. 2108C2a-1]Predicted molecular weight: 48 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CARD8 mAb. [Lot No. 2108C2a-1]Predicted molecular weight: 48 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CARD8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2108C2a-1])

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb. [Lot No. 2108C2a-1])

Related Product Information for anti-CARD8 antibody

Caspase recruitment domain (CARD)-containing proteins, such as CARD8, are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB; see MIM 164011) in the context of apoptosis or inflammation, respectively (Bouchier-Hayes et al., 2001).[supplied by OMIM]. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 296531358
• NCBI GeneID: 22900
• NCBI Accession #: NP_055774.2
• NCBI GenBank Nucleotide #: NM_014959
• NCBI Official Full Name: Homo sapiens caspase recruitment domain family, member 8 (CARD8), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 8
• NCBI Official Symbol: CARD8
• NCBI Official Synonym Symbols: NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• NCBI Protein Information: caspase recruitment domain-containing protein 8; CARD inhibitor of NF-kappaB-activating ligands; apoptotic protein NDPP1; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
• UniProt Protein Name: Caspase recruitment domain-containing protein 8
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD8
• UniProt Entry Name: CARD8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; NACHT domain binding; protein homodimerization activity; caspase activator activity

Biological Process: caspase activation; positive regulation of caspase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion