Mouse anti-Human TFAP2A Monoclonal Antibody | anti-TFAP2A antibody

Mouse monoclonal antibody Anti-Human TFAP2A

Cat. Num. AAA120100

• Gene Names: TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• Reactivity: Human
• Applications: Dot Blot, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: TFAP2A; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TFAP2A; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); AP-2; AP2TF; TFAP2; AP-2alpha; anti-TFAP2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 959C2a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 3300

• Applicable Applications for anti-TFAP2A antibody: Dot Blot (DB), Western Blot (WB), Flow Cytometry (FC/FACS)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of TFAP2A by Western blot.Samples: Whole cell lysate from human HEK293 (H, 50 ug) and mouse NIH3T3 (M, 50 ug) cells. [Lot No. 959C2a-1]Predicted molecular weight: 48 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TFAP2A mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 959C2a-1])

Related Product Information for anti-TFAP2A antibody

AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM] [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 109389359
• NCBI GeneID: 7020
• NCBI Accession #: NP_003211.1
• NCBI GenBank Nucleotide #: NM_003220
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: Homo sapiens transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• NCBI Official Symbol: TFAP2A
• NCBI Official Synonym Symbols: AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
• NCBI Protein Information: transcription factor AP-2-alpha; AP-2 transcription factor; AP2-alpha; activating enhancer-binding protein 2-alpha; activator protein 2
• UniProt Protein Name: Transcription factor AP-2-alpha
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2A
• UniProt Entry Name: AP2A_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; chromatin binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of epidermal growth factor receptor signaling pathway; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; oculomotor nerve formation; positive regulation of neuron apoptosis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of neuron apoptosis; kidney development; trigeminal nerve development; inner ear morphogenesis; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; embryonic body morphogenesis; forebrain neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of cell migration

Disease: Branchiooculofacial Syndrome