Mouse anti-Human ATP5A Monoclonal Antibody | anti-ATP5A antibody

ATP5A Mouse mAb

Cat. Num. AAA435146

• Gene Names: ATP5PF; F6; CF6; ATP5; ATPM; ATP5A; ATP5J
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: ATP5A; Monoclonal Antibody; ATP5A Mouse mAb; alpha subunit; ATP synthase; ATP synthase alpha chain; mitochondrial; ATP synthase alpha subunit; ATP synthase subunit alpha; H+ transporting; mitochondrial F1 complex; isoform 1; cardiac muscle; isoform 2; non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5AL2; ATPM; epididymis secretory sperm binding protein Li 123m; hATP1; mitochondrial ATP synthetase; oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2; MOM2; OMR; ORM; anti-ATP5A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 108

• Applicable Applications for anti-ATP5A antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of SERPING1, C1IN, C1NH.
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

(ATPA was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25203 of 10 ug. Western blot was performed from the immunoprecipitate using M25203 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction. Blocking and dilution buffer and concentration:5% milk/TBST.)

Immunoprecipitation (IP)

(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling ATPA with M25203 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS)

(All lanes : ATPA Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 59 KDaObserved band size : 59 KDaBlocking/Dilution buffer : 1x TBST.)

Related Product Information for anti-ATP5A antibody

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Product Categories/Family for anti-ATP5A antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 998072088
• NCBI GeneID: 522
• NCBI Accession #: NP_001307196.1
• NCBI GenBank Nucleotide #: NM_001320267.1
• UniProt Accession #: P18859; J3KQ83
• Molecular Weight: Predicted MW: 59kDa
• NCBI Official Full Name: ATP synthase-coupling factor 6, mitochondrial isoform a
• NCBI Official Synonym Full Names: ATP synthase peripheral stalk subunit F6
• NCBI Official Symbol: ATP5PF
• NCBI Official Synonym Symbols: F6; CF6; ATP5; ATPM; ATP5A; ATP5J
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• UniProt Gene Name: ATP5PF
• UniProt Synonym Gene Names: ATPase subunit F6

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.