Mouse anti-Human ACBD5 Monoclonal Antibody | anti-ACBD5 antibody

ACBD5 Mouse mAb

Cat. Num. AAA435111

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: ACBD5; Monoclonal Antibody; ACBD5 Mouse mAb; Acyl-CoA-binding domain-containing protein 5; acyl-Coenzyme A binding domain containing 5; endozepine-related protein; membrane-associated diazepam binding inhibitor; KIAA1996; anti-ACBD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 490

• Applicable Applications for anti-ACBD5 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

(All lanes : ACBD5 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.)

Immunoprecipitation (IP)

(ACBD5 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25019 of 10 ug. Western blot was performed from the immunopre-cipitate using M25019 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ACBD5 with M25019 at 1/200 dilution (blue) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, red). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-ACBD5 antibody

This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-ACBD5 antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 109689720
• NCBI GeneID: 91452
• NCBI Accession #: NP_001035938.1
• NCBI GenBank Nucleotide #: NM_001042473.3
• UniProt Accession #: Q5T8D3; Q5T8D4; Q5T8E1; Q5T8E2; Q86UV1; Q8N6E3; Q9UFB5; B3KQ56; D3DRW0
• Molecular Weight: Predicted MW: 60kDa
• NCBI Official Full Name: acyl-CoA-binding domain-containing protein 5 isoform 2
• NCBI Official Synonym Full Names: acyl-CoA binding domain containing 5
• NCBI Official Symbol: ACBD5
• NCBI Protein Information: acyl-CoA-binding domain-containing protein 5
• UniProt Protein Name: Acyl-CoA-binding domain-containing protein 5
• Protein Family: Acyl-CoA-binding domain-containing protein
• UniProt Gene Name: ACBD5
• UniProt Synonym Gene Names: KIAA1996

NCBI Description

This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.