Western Blot (WB)
Mouse anti-Human ATP synthase subunit gamma Monoclonal Antibody | anti-ATP5C antibody
ATP synthase subunit gamma Mouse mAb
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
ATP synthase subunit gamma; Monoclonal Antibody; ATP synthase subunit gamma Mouse mAb; anti-ATP5C antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Applicable Applications for anti-ATP5C antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
IP: 10 tests
FC/FACS: 1:100-1:200
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ATP5C1.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Immunoprecipitation (IP)
(ATP5C1 was immunoprecipitated from 1mg of 1G2 cells membrane fraction, blotted with M25352 of 10 ug. Western blot was performed from the immunoprecipitate using M25352 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1:1G2 cells membrane fraction. Lane2: IP product of 1G2 cells membrane fraction.Predicted band size : 33 KDaObserved band size : 33 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(ATP5C1 was immunoprecipitated from 1mg of 1G2 cells membrane fraction, blotted with M25352 of 10 ug. Western blot was performed from the immunoprecipitate using M25352 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1:1G2 cells membrane fraction. Lane2: IP product of 1G2 cells membrane fraction.Predicted band size : 33 KDaObserved band size : 33 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed 1G2 cells labeling ATP5C1 with M25352 at 1/200 dilution (blue) compared with a mouse monoclonal IgG isotype control (black). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed 1G2 cells labeling ATP5C1 with M25352 at 1/200 dilution (blue) compared with a mouse monoclonal IgG isotype control (black). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Related Product Information for anti-ATP5C antibody
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.
Product Categories/Family for anti-ATP5C antibody
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Product Notes
The ATP5C (Catalog #AAA435251) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATP synthase subunit gamma Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP synthase subunit gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the ATP5C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP synthase subunit gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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