Goat anti-Human Receptor Interacting Protein 140 Polyclonal Antibody | anti-Nrip1 antibody

Receptor Interacting Protein 140 (RIP140, RIP-140, Nuclear Factor RIP-140, Nuclear Factor RIP140, Nuclear Receptor Interacting Protein 1, NRIP1)

Cat. Num. AAA619448

• Gene Names: Nrip1; RIP140; AA959574; AW456757; 9630050P12; 6030458L20Rik; 8430438I05Rik
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Receptor Interacting Protein 140; Polyclonal Antibody; Receptor Interacting Protein 140 (RIP140; RIP-140; Nuclear Factor RIP-140; Nuclear Factor RIP140; Nuclear Receptor Interacting Protein 1; NRIP1); Anti -Receptor Interacting Protein 140 (RIP140; anti-Nrip1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RIP140. Sequence Homology: mouse, rat, bovine, canine..
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

• Applicable Applications for anti-Nrip1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000
• Immunogen: Synthetic peptide corresponding to C-EKDSPRLTKTNP from the internal region of human RIP140 (NP_003480.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(EB09546 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue > P114-RHO-GEF to ZNF9 > Goat Anti-RIP140 Antibody)

Related Product Information for anti-Nrip1 antibody

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor-Interacting Protein 140, also known as RIP140, has been shown to enhance estrogen receptor transcriptional activity through the AF2-AD domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPARa) and has been proposed to competitively inhibit PPARa interaction with another known nuclear receptor coactivator, SRC-1. However, the specific roles for coactivation and corepression by RIP140 in steroid receptor pathways is currently not well understood.

Product Categories/Family for anti-Nrip1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 3820494
• NCBI GeneID: 268903
• UniProt Accession #: Q8CBD1; Q3U166; Q8C3Y8; Q9Z2K2
• Molecular Weight: 126,337 Da
• NCBI Official Full Name: receptor interacting protein 140
• NCBI Official Synonym Full Names: nuclear receptor interacting protein 1
• NCBI Official Symbol: Nrip1
• NCBI Official Synonym Symbols: RIP140; AA959574; AW456757; 9630050P12; 6030458L20Rik; 8430438I05Rik
• NCBI Protein Information: nuclear receptor-interacting protein 1; nuclear factor RIP140; receptor-interacting protein 140
• UniProt Protein Name: Nuclear receptor-interacting protein 1
• Protein Family: Nuclear receptor-interacting protein
• UniProt Gene Name: Nrip1
• UniProt Entry Name: NRIP1_MOUSE

Uniprot Description

RIP140: Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH. Interacts with ZNF366.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; histone deacetylase complex; nucleolus; nuclear speck; cell junction; nucleus

Molecular Function: retinoid X receptor binding; protein binding; retinoic acid receptor binding; histone deacetylase binding; transcription coactivator activity; transcription cofactor activity; estrogen receptor binding; transcription corepressor activity; receptor binding; glucocorticoid receptor binding; nuclear hormone receptor binding

Biological Process: ovulation; regulation of transcription from RNA polymerase II promoter; sequestering of lipid; regulation of transcription, DNA-dependent; transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle rupture