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2934 results for "Western Blot Reagents" - showing 1-50
Western Blot (WB)
(Western Blot: Lysates were prepared from human liver in reducing or non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to a PVDF membrane. The blot was developed with 2ug/ml of MBS604737 and chemiluminescent detection substrate.)
a1-Acid Glycoprotein, Monoclonal Antibody (Cat# AAA604737)
Full Name
a1-Acid Glycoprotein (Orosomucoid)
Gene Names
Orm1; AGP; OMD; Orm; Agpa1
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Pricing
$800/0.1 mg | $3,590/5x0.1 mg
Alpha Fetoprotein (AFP) Antigen Grade, Native Protein (Cat# AAA318070)
Full Name
Human AFP Antigen Grade
Gene Names
AFP; FETA; HPAFP; AFP
Applications
Western Blot
Purity
>95% pure (PAGE electrophoresis, Western blot)
Product is 0.2um filtered.
Product is 0.2um filtered.
Pricing
$855/1 mg | $3,705/5x1 mg
Blocking Buffer for ELISA, Reagent (Cat# AAA318404)
Full Name
Blocking Buffer for ELISA
Applications
Suitable for use in ELISA and Western Blot.
Pricing
$325/500 mL | $1,305/5x500 mL
Cancer Antigen CA125 (Ovarian Cancer) Antigen Grade, Native Protein (Cat# AAA318371)
Full Name
Human CA125 Antigen Grade
Applications
Suitable for use in in Western Blot and RIA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Purity
Known contaminants:
CA19-9 = 207.5 Units/mL (<0.11%) (Elecsys)
CA15-3 = 2.19 Units/mL (<0.1%) (Elecsys)
Product is 0.2um filtered.
CA19-9 = 207.5 Units/mL (<0.11%) (Elecsys)
CA15-3 = 2.19 Units/mL (<0.1%) (Elecsys)
Product is 0.2um filtered.
Pricing
$675/50K Units | $2,880/5x50K Units
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Alpha Tubulin F2C HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Alpha-Tubulin, Monoclonal Recombinant Antibody (Cat# AAA488244)
Full Name
Anti-Alpha-Tubulin [F2C]
Reactivity
Human
Applications
EIA, WB
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(100 ng of Ebola Virus VP40 protein (MBS5304547) was loaded, antibody dilution was 1:3000)
Ebola Virus VP40, Recombinant Protein (Cat# AAA5304547)
Full Name
Ebola Virus VP40 protein
Applications
EIA, WB
Purity
> 90% pure
Purified using proprietary chromatographic techniques
Purified using proprietary chromatographic techniques
Pricing
$260/0.1 mg | $980/5x0.1 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD38, Monoclonal Recombinant Antibody (Cat# AAA488298)
Full Name
Anti-CD38 [AT13/5]
Gene Names
CD38; ADPRC1; ADPRC 1
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
( Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.)
amyloid beta, Monoclonal Recombinant Antibody (Cat# AAA488264)
Full Name
Anti-amyloid beta [6E10]
Reactivity
Human
Applications
IHC, EIA, WB, IP
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-MUC1 antibody HMFG2 MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of HMFG2 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
MUC1, Monoclonal Recombinant Antibody (Cat# AAA488262)
Full Name
Anti-MUC1 [HMFG2]
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
IHC
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-p53 antibody Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
p53, Monoclonal Recombinant Antibody (Cat# AAA488222)
Full Name
Anti-p53 [PAb421]
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Monkey, Mouse, Rabbit, Rat
Applications
EIA, IF, FC/FACS, IP, WB
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Alpha-fetoprotein AF5 HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of AF5 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Alpha-fetoprotein (AFP), Monoclonal Recombinant Antibody (Cat# AAA488236)
Full Name
Anti-Alpha-fetoprotein (AFP) [AF5]
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR antibody 528 HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 528 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analysed on a FACSCanto flow-cytometer.)
EGFR, Recombinant Antibody (Cat# AAA488090)
Full Name
Anti-EGFR [528]
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
IF, IHC, IP, WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
ED-B domain of fibronectin, Monoclonal Recombinant Antibody (Cat# AAA488256)
Full Name
Anti-ED-B domain of fibronectin [G4]
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-MUC1 SM3 MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of SM3 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
MUC1, Monoclonal Recombinant Antibody (Cat# AAA488238)
Full Name
Anti-MUC1 [SM3]
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
EIA, IHC, FC/FACS
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (MBS488423) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488423, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Cardiac Troponin I, Monoclonal Recombinant Antibody (Cat# AAA488423)
Full Name
Anti-Cardiac Troponin I [scFv 180]
Gene Names
TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
Reactivity
Human
Applications
WB, SPR
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD4 antibody MT310 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of MT310 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD4, Recombinant Antibody (Cat# AAA488164)
Full Name
Anti-CD4 [MT310]
Gene Names
CD4; CD4mut
Reactivity
Human
Applications
WB, IP, IF, IHC, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
( Binding curve of anti-Bet v 1 antibody BV16 to Bet v 1 ELISA Plate coated with Bet v 1-A (RayBiotech, 228-22389) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used. as also observed for This is suggestive of two binding sites on Bet v 1-A for which has differing affinities.)
Bet v 1, Monoclonal Recombinant Antibody (Cat# AAA488338)
Full Name
Anti-Bet v 1 [BV16]
Reactivity
Birch (Betula pendula)
Applications
WB, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ED-B domain of fibronectin, Monoclonal Recombinant Antibody (Cat# AAA488255)
Full Name
Anti-ED-B domain of fibronectin [G4]
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC754).)
Mitochondria, Monoclonal Antibody (Cat# AAA438265)
Full Name
Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human.
Shows broad species reactivity including insects or bacteria
Shows broad species reactivity including insects or bacteria
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Mitochondria Monoclonal Antibody (AE-1).)
Mitochondria, Monoclonal Antibody (Cat# AAA439479)
Full Name
Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
EIA, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488486)
Full Name
Anti-TIGIT [1B4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
EIA, FC/FACS, BL
Purity
Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD74, Monoclonal Recombinant Antibody (Cat# AAA488365)
Full Name
Anti-CD74 [In-1]
Reactivity
Mouse
Applications
IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).)
Golgi Complex, Monoclonal Antibody (Cat# AAA438508)
Full Name
Golgi Complex (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IF, WB, ICC, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-HLA class II antibody F3.3 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of F3.3 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
HLA class II, Monoclonal Recombinant Antibody (Cat# AAA488234)
Full Name
Anti-HLA class II [F3.3]
Reactivity
Human
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 (MBS488424) followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) MBS488424 with Alexa Fluor 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
GCN4, Monoclonal Recombinant Antibody (Cat# AAA488424)
Full Name
Anti-GCN4 [C11L34]
Gene Names
GCN4; AAS101; AAS3; ARG9
Reactivity
S. cerevisiae
Applications
WB, SPR, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
( Immunohistochemical staining of guinea pig skin using anti-CD4 antibody CE9.1 (Clenoliximab) Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) was used to stain samples at a concentration of 3 ug/ml.)
CD4, Recombinant Antibody (Cat# AAA488199)
Full Name
Anti-CD4 [CE9.1 (Clenoliximab)]
Gene Names
CD4; CD4mut
Reactivity
Human, Chimpanzee
Applications
EIA, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD34 antibody My10 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of My10 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD34, Monoclonal Recombinant Antibody (Cat# AAA488220)
Full Name
Anti-CD34 [My10]
Reactivity
Human
Applications
FC/FACS, IP, IHC
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
( Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.)
Lymphotoxin alpha, Monoclonal Recombinant Antibody (Cat# AAA488355)
Full Name
Anti-Lymphotoxin alpha [3F12.2D3]
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Reactivity
Human
Applications
EIA, FC/FACS, SPR
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescent staining of rat thymus using anti-CD25 antibody Formaldehyde-fixed rat thymus slices were stained with at 5 ug/ml and detected with a FITC-conjugated secondary antibody. shows weak labelling of rat cells by IF.)
IL-2R alpha (CD25), Recombinant Antibody (Cat# AAA488159)
Full Name
Anti-IL-2R alpha (CD25) [Basiliximab]
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human
Applications
FC/FACS, IF, IHC, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719))
Mitochondria, Monoclonal Antibody (Cat# AAA438160)
Full Name
Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human.
Shows broad species reactivity.
Does not react with insects or bacteria
Shows broad species reactivity.
Does not react with insects or bacteria
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
His tag, Recombinant Antibody (Cat# AAA488031)
Full Name
Anti-His tag (C-term) [3D5]
Applications
WB, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
DARC, Monoclonal Recombinant Antibody (Cat# AAA488374)
Full Name
Anti-DARC [2C3]
Reactivity
Human
Applications
EIA, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fas antibody R-125224 (Ab00802) Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (Ab00802-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fas, Monoclonal Recombinant Antibody (Cat# AAA488362)
Full Name
Anti-Fas [R-125224]
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
HIV-1 tat, Strain IIIB, Active Protein (Cat# AAA636668)
Full Name
HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Applications
Suitable for use in T Cell Research, ELISA and Western Blot.
Other applications not been tested.
Other applications not been tested.
Purity
Purified by ion affinity and reverse phase HPLC to ? 99% as determined by SDS-PAGE and HPLC.
Pricing
$870/0.05 mg | $3,905/5x0.05 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR antibody Matuzumab HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
EGFR, Recombinant Antibody (Cat# AAA488209)
Full Name
Anti-EGFR [Matuzumab]
Reactivity
Human
Applications
FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed U251 cells with anti-Prion antibody 3F4 Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 (MBS488429) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488429, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Prion, Monoclonal Recombinant Antibody (Cat# AAA488429)
Full Name
Anti-Prion [3F4]
Reactivity
Hamster, Human
Applications
EIA, WB, IHC, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD55, Monoclonal Recombinant Antibody (Cat# AAA488248)
Full Name
Anti-CD55 [LU30]
Gene Names
CD55; CR; TC; DAF; CROM; CHAPLE
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-human IgG4 Fc-PE (MBS674080))
Mouse Anti-Human IgG4 (gamma 4 chain specific), Monoclonal Secondary Antibody (Cat# AAA674079)
Full Name
Mouse Anti-Human IgG4 (Fc)-BIOT
Reactivity
Human
Pricing
$270/0.5 mg | $1,070/5x0.5 mg
HIV-1 gag, p24, Strain IIIB, Active Protein (Cat# AAA635672)
Full Name
HIV-1 gag, p24, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Applications
T-cell Research, ELISA and Western Blot
Other applications not tested.
Other applications not tested.
Purity
Purified by cation/anion affinity and gel exclusion chromatography >98% purity as determined by SDS-PAGE, reduced.
Pricing
$870/0.05 mg | $3,905/5x0.05 mg
Valpha TCR, Recombinant Antibody (Cat# AAA488206)
Full Name
Anti-Valpha TCR [34]
Reactivity
Human
Applications
EIA, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Basiliximab Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Basiliximab at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
IL-2R alpha (CD25), Recombinant Antibody (Cat# AAA488160)
Full Name
Anti-IL-2R alpha (CD25) [Basiliximab]
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human
Applications
FC/FACS, IF, IHC, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed HepG2 cells with anti-Serotransferrin antibody TW4.20 Immunofluorescence analysis of paraformaldehyde fixed HpeG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of TW4.20 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing diffuse cytoplasmic staining, likely from protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Transferrin, Monoclonal Recombinant Antibody (Cat# AAA488340)
Full Name
Anti-Transferrin [TW4.20]
Gene Names
TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
Reactivity
Human
Applications
FC/FACS, EIA, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Discoidin Domain Receptor 1, Monoclonal Recombinant Antibody (Cat# AAA488317)
Full Name
Anti-Discoidin Domain Receptor 1 [3E3]
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human
Applications
EIA, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Beta Tubulin S11B MCF-7 (A) and HeLa (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S11B at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Beta-Tubulin, Monoclonal Recombinant Antibody (Cat# AAA488246)
Full Name
Anti-Beta-Tubulin [S11B]
Reactivity
Human
Applications
EIA, WB
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
LAMP1 , Monoclonal Antibody (Cat# AAA2544199)
Full Name
Rabbit anti Human LAMP1 Monoclonal Antibody
Gene Names
LAMP1; LAMPA; CD107a; LGP120
Reactivity
Human
Applications
WB, EIA, IHC, ICC, IF, IP, FC/FACS
Pricing
$790/0.2 mL | $510/0.12 mL | $325/0.06 mL
Western Blot (WB)
( Western Blot using anti-IL-2 antibody LNKB-2 Untreated human peripheral blood monocyte (PBM) (A), PMA/lonomycin-treated PBM (B), human spleen (C), human thymus (D), Jurkat cell (E) and Molt4 cell (F) lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of LNKB-2 at 1 ug/ml (A, B) or 0.1 ug/ml (C-F) before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL-2 is 17.6kDa. successfully detected Il-2 in multiple human tissue / cell samples.)
IL-2, Monoclonal Recombinant Antibody (Cat# AAA488292)
Full Name
Anti-IL-2 [LNKB-2]
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
WB, IP, IF, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD38 antibody HB7 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of HB7 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD38, Recombinant Antibody (Cat# AAA488084)
Full Name
Anti-CD38 [HB7]
Gene Names
CD38; ADPRC1; ADPRC 1
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
DDDDK-tag, Monoclonal Recombinant Antibody (Cat# AAA488278)
Full Name
Anti-DDDDK-tag [M2.1]
Applications
IF, WB, EIA
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody 3A1E Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD7, Monoclonal Recombinant Antibody (Cat# AAA488295)
Full Name
Anti-CD7 [3A1E]
Gene Names
CD7; GP40; TP41; Tp40; LEU-9
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
( Immunohistochemical staining of human lung cancer using anti-Bax antibody Formalin fixed human lung cancer slices were were stained with a at 3 ug/ml.)
Bax, Monoclonal Recombinant Antibody (Cat# AAA488281)
Full Name
Anti-Bax (active monomer) [6A7]
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
