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Western Blot (WB) (Western Blot: Lysates were prepared from human liver in reducing or non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to a PVDF membrane. The blot was developed with 2ug/ml of MBS604737 and chemiluminescent detection substrate.)

Mouse anti-Human a1-Acid Glycoprotein Monoclonal Antibody | anti-Orm1 antibody

a1-Acid Glycoprotein (Orosomucoid)

Gene Names
Orm1; AGP; OMD; Orm; Agpa1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Synonyms
a1-Acid Glycoprotein; Monoclonal Antibody; a1-Acid Glycoprotein (Orosomucoid); Anti -a1-Acid Glycoprotein (Orosomucoid); anti-Orm1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9H10
Specificity
Recognizes human Alpha-1 acid glycoprotein.
Purity/Purification
Purified by Protein A affinity chromatography from culture supernatant.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5 mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Orm1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 2ug/ml using human liver tissue
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin embedded sections of human liver.
Optimal dilutions to be determined by the researcher
Immunogen
Human AGP from plasma.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Reconstituted product is stable at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: Lysates were prepared from human liver in reducing or non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to a PVDF membrane. The blot was developed with 2ug/ml of MBS604737 and chemiluminescent detection substrate.)

Western Blot (WB) (Western Blot: Lysates were prepared from human liver in reducing or non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to a PVDF membrane. The blot was developed with 2ug/ml of MBS604737 and chemiluminescent detection substrate.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of alpha 1-Acid Glycoprotein in immersion fixed paraffin-embedded sections of human liver using MBS604737 (25ug/ml) overnight at 4°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of alpha 1-Acid Glycoprotein in immersion fixed paraffin-embedded sections of human liver using MBS604737 (25ug/ml) overnight at 4°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.)
Related Product Information for anti-Orm1 antibody
Alpha-1 acid glycoprotein (AGP), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP levels are elevated during inflammatory responses. AGP binds a wide range of molecules in the circulation. Human and mouse AGP share 43% amino acid sequence identity.
Product Categories/Family for anti-Orm1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-1-acid glycoprotein
NCBI Official Synonym Full Names
orosomucoid 1
NCBI Official Symbol
Orm1
NCBI Official Synonym Symbols
AGP; OMD; Orm; Agpa1
NCBI Protein Information
alpha-1-acid glycoprotein
UniProt Protein Name
Alpha-1-acid glycoprotein
Protein Family
UniProt Gene Name
Orm1
UniProt Entry Name
A1AG_RAT

NCBI Description

an acute phase reactant protein; may have a role in the acute inflammation response [RGD]

Uniprot Description

ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space

Molecular Function: drug binding

Biological Process: response to drug; response to vitamin E; organ regeneration; regulation of immune system process; transport; acute-phase response; response to lipopolysaccharide; chronic inflammatory response to antigenic stimulus; response to organic cyclic substance; acute inflammatory response to antigenic stimulus

Research Articles on Orm1

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Product Notes

The Orm1 orm1 (Catalog #AAA604737) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The a1-Acid Glycoprotein (Orosomucoid) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's a1-Acid Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 2ug/ml using human liver tissue Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin embedded sections of human liver. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Orm1 orm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a1-Acid Glycoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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