Mouse Mitochondria Monoclonal Antibody

Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody

Cat. Num. AAA438160

• Reactivity: Human.; Shows broad species reactivity.; Does not react with insects or bacteria
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Mitochondria; Monoclonal Antibody; Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody; anti-Mitochondria antibody

• Host: Mouse
• Reactivity: Human.; Shows broad species reactivity.; Does not react with insects or bacteria
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: MTC719
• Specificity: This MAb recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

• Applicable Applications for anti-Mitochondria antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.25-0.5ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Mitochondria in Cytoplasm
• Immunogen: Mitochondrial fraction of HeLa cells
• Positive Control: HeLa or HepG2 cells. Hepatic carcinomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody is stable for 24 months.,Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (MTC719).)