HLA class II Recombinant Antibody | HLA-DR recombinant antibody

Anti-HLA class II [F3.3]

Cat. Num. AAA488234

• Reactivity: Human
• Purity: Purified; Protein A Affinity Purified
• Synonyms: HLA class II; Monoclonal Recombinant Antibody; Anti-HLA class II [F3.3]; HLA class II related epitope; HLA-DR recombinant antibody

• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, kappa
• Clone Number: F3.3
• Specificity: Recognising anti-class II HLA molecules.
• Purity/Purification: Purified; Protein A Affinity Purified

• Antigen: HLA class II
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Publication Note: Describes the preparation and characterisation of anti human HLA class II antibody by of ADCC ofG-CSF primed PMN expressing in the presence of F3.3 to HLA class II related epitope (HLA-DR).
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Flow Cytometry (FC/FACS)

( Flow-cytometry using anti-HLA class II antibody F3.3 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of F3.3 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

Flow Cytometry (FC/FACS)

( Flow-cytometry using the anti-HLA II DR antibody F3.3 Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F3.3 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)

Western Blot (WB)

( Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3 Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F3.3 at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.)

Related Product Information for HLA-DR recombinant antibody

Recombinant monoclonal antibody to HLA class II. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F3.3.

Product Categories/Family for HLA-DR recombinant antibody

Immunology; Infectious disease

References

Elsässer D, Valerius T, Repp R, Weiner GJ, Deo Y, Kalden JR, van de Winkel JG, Stevenson GT,Glennie MJ, Gramatzki M. Blood. 1996 May 1;87(9):3803-12. PMID:8611706