Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-HLA class II antibody F3.3 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of F3.3 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
HLA class II Recombinant Antibody | HLA-DR recombinant antibody
Anti-HLA class II [F3.3]
Protein A Affinity Purified
Protein A Affinity Purified
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-HLA class II antibody F3.3 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of F3.3 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-HLA class II antibody F3.3 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of F3.3 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-HLA II DR antibody F3.3 Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F3.3 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-HLA II DR antibody F3.3 Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F3.3 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Western Blot (WB)
( Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3 Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F3.3 at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.)
Western Blot (WB)
( Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3 Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F3.3 at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.)
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Product Notes
The HLA-DR (Catalog #AAA488234) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA class II [F3.3] reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "HLA class II, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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