Alpha Fetoprotein (AFP) Antigen Grade Native Protein | AFP native protein
Human AFP Antigen Grade
Product is 0.2um filtered.
Product is 0.2um filtered.
Total Protein: 3.52 mg/mL(OD280nm,E0.1%= 0.53) (varies by lot)
Human Alpha Fetoprotein (AFP) Antigen Grade
AFP has three isoforms (AFP-L1, AFP-L2 and AFP-L3) of which AFP-L3 is the main isoform associated with germ cell and liver tumors. MBS318070 is derived from a liver tumor cell (not human cord serum) and contains approximately 70% of the AFP-L3 isoform (compared to human cord serum which typically only contains 10% AFP-L3).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]
Uniprot Description
Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form.
Subcellular location: Secreted.
Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.
Developmental stage: Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
Post-translational modification: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.
Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.
Research Articles on AFP
2. AFP promoter enhancer increased specific expression of the human sodium iodide symporter (hNIS) for targeted radioiodine therapy of hepatocellular carcinoma.
3. Alpha-fetoprotein was more sensitive than des-gamma carboxyprothrombin and lectin-bound alpha-fetoprotein in diagnosing early hepatocellular carcinoma.
4. Case Report: Alpha-fetoprotein (AFP)-producing ovarian tumor in an elderly woman.
5. The utility of the tumour markers carcinoembryonic antigen, cancer antigen 125, carbohydrate antigen 19-9, carbohydrate antigen 15-3, alpha-fetoprotein and human chorionic gonadotropin for the diagnosis solitary pulmonary nodules, was investigated.
6. Case Report: Alpha-fetoprotein expressing metastastic adenocarcinoma of the esophago-gastric junction responding favorably to capecitabine and oxaliplatin.
7. small amplitude undulations in AFP are associated with recurrence of germ cell neoplasm
8. Low Alphafetoprotein levels and increased CA 19-9 levels can be used as tumor markers in diagnosis of molar pregnancies in conjunction with clinical and sonographic findings and serum levels of human chorionic gonadotropin
9. Report des-gamma-carboxyprothrombin, alpha-fetoprotein and AFP-L3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma.
10. These data support the idea that ZHX2 contributes to AFP repression in the liver after birth and may also be involved in AFP reactivation in liver cancer.
11. In hepatocellular carcinoma, pre-transplant AFP levels >400 ng/ml were associated with higher tumor recurrence.
12. Serum samples were investigated for carcinoembryonic antigen (CEA), CA125 and MUC1, alpha-foetoprotein, neuron-specific enolase and CA19.9.
13. In patients with hepatocellular carcinoma, a high frequency of AFP-specific CD8(+) T cells directed against different epitopes suggest that AFP has a strong and broad immunogenicity.
14. Molecular background of AFP in liver cancer cells as revealed by global RNA expression analysis is reported.
15. results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via dysfunction of the p53/Bax/cytochrome c/caspase-3 signaling pathway in AFP
16. Elevated alpha-fetoprotein is associated with primary central nervous system germ cell tumors.
17. Results indicate that the poor prognosis associated with high alpha-fetoprotein in hepatocellular carcinoma is due to high cell proliferation, high angiogenesis, and low apoptosis.
18. targeting of AFP with siRNA could be a potential therapeutic approach for hepatoma
19. substitution of aa 70 of HCV-1b core region is an important predictor of elevated AFP in non-HCC (hepatocellular carcinoma) patients.
20. The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed.
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Product Notes
The AFP afp (Catalog #AAA318070) is a Native Protein produced from Cell culture derived; human hepatocellular carcinoma. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha Fetoprotein (AFP) Antigen Grade can be used in a range of immunoassay formats including, but not limited to, Western Blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Fetoprotein (AFP) Antigen Grade, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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