Immunofluorescence (IF)
(Immunofluorescence staining of fixed U251 cells with anti-Prion antibody 3F4 Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 (MBS488429) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488429, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Prion Recombinant Antibody
Anti-Prion [3F4]
Reactivity
Hamster, Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A Affinity Purified
Synonyms
Prion; Monoclonal Recombinant Antibody; Anti-Prion [3F4]; Major prion protein; p27-30; CD30 antigen; prion protein PrP; prion-related protein; Prion recombinant antibody
Reactivity
Hamster, Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
3F4
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for Prion recombinant antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Immunogen
Hamster 263K PrPs.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
The antibody binds specifically to prion, a misfolded protein that has the ability to enter healthy organisms and cause other misfolding of other proteins into their prion form. This plays an essential role in the pathogenesis of certain neurodegenerative disorders such as Creutzfeldt-Jakob disease (CJD), Gerstmann-Staussler syndrome (GSS) and bovine spongiform encephalopathy (BSE). These disorders are characterised by an accumulation of prion proteins in the brain, which have a disrupted secondary structure.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed U251 cells with anti-Prion antibody 3F4 Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 (MBS488429) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488429, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed U251 cells with anti-Prion antibody 3F4 Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 (MBS488429) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488429, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for Prion recombinant antibody
Recombinant monoclonal antibody to Prion. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 3F4.
Product Categories/Family for Prion recombinant antibody
References
Kascsak RJ, Rubenstein R, Merz PA, Tonna-DeMasi M, Fersko R, Carp RI, Wisniewski HM, Diringer H. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J Virol. 1987 Dec;61(12):3688-93. PMID:2446004
Original Published Journal: J Virol. 1987 Dec;61(12):3688-93.
Original Published Journal: J Virol. 1987 Dec;61(12):3688-93.
Publication Note: Describes the generation a species-specific monoclonal antibody directed against protease-resistant proteins (PrPs) in order to study the relationship of PrPs to their host precursor protein and to scrapie-associated fibril proteins.
Original Published Journal: J Virol. 1987 Dec;61(12):3688-93.
Original Published Journal: J Virol. 1987 Dec;61(12):3688-93.
Publication Note: Describes the generation a species-specific monoclonal antibody directed against protease-resistant proteins (PrPs) in order to study the relationship of PrPs to their host precursor protein and to scrapie-associated fibril proteins.
Similar Products
Product Notes
The Prion (Catalog #AAA488429) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Prion [3F4] reacts with Hamster, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prion can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Prion for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prion, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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