Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Alpha Tubulin F2C HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Alpha-Tubulin Recombinant Antibody | TUBA recombinant antibody
Anti-Alpha-Tubulin [F2C]
Protein A Affinity Purified
Protein A Affinity Purified
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Alpha Tubulin F2C HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Alpha Tubulin F2C HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-Alpha-tubulin antibody F2C Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-Alpha-tubulin antibody F2C Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of human cerebral cortex tissue using anti-Alpha Tubulin antibody F2C Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.)
Immunohistochemistry (IHC)
( Immunohistochemical staining of human cerebral cortex tissue using anti-Alpha Tubulin antibody F2C Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.)
Western Blot (WB)
( Western Blot using anti-Alpha-Tubulin antibody F2C HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-Tubulin is ~55kDa. HEK293, and HepG2 cell lysate samples.)
Western Blot (WB)
( Western Blot using anti-Alpha-Tubulin antibody F2C HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-Tubulin is ~55kDa. HEK293, and HepG2 cell lysate samples.)
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Product Notes
The TUBA (Catalog #AAA488244) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha-Tubulin [F2C] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the TUBA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Tubulin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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