HIV-1 gag, p24, Strain IIIB Active Protein
HIV-1 gag, p24, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Applications
ELISA, Western Blot
Purity
Purified by cation/anion affinity and gel exclusion chromatography >98% purity as determined by SDS-PAGE, reduced.
Synonyms
HIV-1 gag; p24; Strain IIIB; Recombinant (Human Immunodeficiency Virus-1) (Biotin); Strain IIIB active protein
Host
Baculovirus
Specificity
Binds to anti-p24 (monoclonal) and polyclonal antibodies in ELISA and Western Blot as determined by strepavidin-AP/HRP assays.
Purity/Purification
Purified by cation/anion affinity and gel exclusion chromatography >98% purity as determined by SDS-PAGE, reduced.
Form/Format
Supplied as a liquid in PBS,pH 8, BSA, 0.02% sodium azide. Labeled with Biotin.
Concentration
1mg/ml (varies by lot)
Applicable Applications for HIV-1 gag, p24, Strain IIIB active protein
T-cell Research, ELISA and Western Blot
Other applications not tested.
Other applications not tested.
Application Notes
ELISA: 1:5000 depending on the concentration of the test ligand and specific and sensitivity of the secondary reagents. p24/Anti-p24 antibody capture ELISA may be performed using 1-10ng antibody in solid phase and 0.1-1ng of MBS635672.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Biological Activity
Binds to solid phase anti-p24 pAb(oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Coupling Ratio
~10-20 moles of biotin per mole p24.
Preparation and Storage
Store at 4°C. Stable for 6 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Categories/Family for HIV-1 gag, p24, Strain IIIB active protein
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Product Notes
The HIV-1 gag, p24, Strain IIIB (Catalog #AAA635672) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV-1 gag, p24, Strain IIIB can be used in a range of immunoassay formats including, but not limited to, T-cell Research, ELISA and Western Blot Other applications not tested. ELISA: 1:5000 depending on the concentration of the test ligand and specific and sensitivity of the secondary reagents. p24/Anti-p24 antibody capture ELISA may be performed using 1-10ng antibody in solid phase and 0.1-1ng of MBS635672. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HIV-1 gag, p24, Strain IIIB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gag, p24, Strain IIIB, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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