Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).)
Mouse anti-Human Golgi Complex Monoclonal Antibody
Golgi Complex (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry
Synonyms
Golgi Complex; Monoclonal Antibody; Golgi Complex (Marker for Human Cells) Mouse Monoclonal Antibody; anti-Golgi Complex antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM581
Specificity
This MAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Applicable Applications for anti-Golgi Complex antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1.0 ug/million cells)
Immunofluorescence (1-2 ug/ml)
Western Blot (1-2 ug/ml)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2 ug/ml for 30 min)
Immunohistochemistry (Formalin-fixed) (1-2 ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mins at 90°C followed by cooling at RT for 20 minutes)
Optimal dilution should be determined by the enduser.
Immunofluorescence (1-2 ug/ml)
Western Blot (1-2 ug/ml)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2 ug/ml for 30 min)
Immunohistochemistry (Formalin-fixed) (1-2 ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mins at 90°C followed by cooling at RT for 20 minutes)
Optimal dilution should be determined by the enduser.
Cellular Localization
Golgi complex in Cytoplasm
Immunogen
SU-DHL-1 large cell lymphoma cells.
Positive Control
Tonsil or lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8°C.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).)
References
Yuasa, K et. al. Binding and Phosphorylation of a Novel Male Germ Cell-specific cGMP-dependent Protein Kinase-anchoring Protein by cGMP-dependent Protein Kinase I. J Biol Chem, 275(7):4897-4905;2000. Yoshio Endo et. al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer science 98;2007
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Product Notes
The Golgi Complex (Catalog #AAA438508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Golgi Complex (Marker for Human Cells) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Golgi Complex can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1.0 ug/million cells) Immunofluorescence (1-2 ug/ml) Western Blot (1-2 ug/ml) Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2 ug/ml for 30 min) Immunohistochemistry (Formalin-fixed) (1-2 ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mins at 90°C followed by cooling at RT for 20 minutes) Optimal dilution should be determined by the enduser. Researchers should empirically determine the suitability of the Golgi Complex for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Golgi Complex, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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