Mouse anti-Human Ig Kappa Light Chain Monoclonal Antibody

Anti-Hu Ig Kappa Light Chain PE-Cy7

Cat. Num. AAA1802228

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: Ig Kappa Light Chain; Monoclonal Antibody; Anti-Hu Ig Kappa Light Chain PE-Cy7; Immunoglobulin kappa; Igk; anti-Ig Kappa Light Chain antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: TB28-2
• Specificity: The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
• Purity/Purification: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-Ig Kappa Light Chain antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: PE-Cy7
• Immunogen: Human IgG-kappa myeloma protein
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig Kappa Light Chain (TB28-2) PE-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human Ig Kappa Light Chain positive B cells (red-filled) from Ig Kappa Light Chain negative CD19 negative (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-Ig Kappa Light Chain antibody

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

References

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*Franklin EC: Structure and Functional Application (FUNC)tion of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690ordinalp