Mouse anti-Human CD23 Monoclonal Antibody | anti-CD23 antibody

Anti-Hu CD23 PE

• Gene Names: FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD23; Monoclonal Antibody; Anti-Hu CD23 PE; FCER2; Fc-epsilon-RII; BLAST-2; FCE2; CLEC4J; IGEBF; low affinity immunoglobulin epsilon Fc receptor; anti-CD23 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: EBVCS-5
• Specificity: The mouse monoclonal antibody EBVCS-5 recognizes an extracellular epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25kDa cleavage sites.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD23 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: EBV-transformed human cells
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD23 (EBVCS-5) PE antibody(10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD23 (EBVCS-5) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD23 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD23 (EBVCS-5) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD23 antibody

CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.

References

*Byrd JC, O'Brien S, Flinn IW, Kipps TJ, Weiss M, Rai K, Lin TS, Woodworth J, Wynne D, Reid J, Molina A, Leigh B, Harris S: Phase 1 study of lumiliximab with detailed pharmacokinetic and pharmacodynamic measurements in patients with relapsed or refractory chronic lymphocytic leukemia. Clin Cancer Res. 2007 Aug 1;13(15 Pt 1):4448-55., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=byrd%20jc%,*Rumi C, Rutella S, Leone G, Bonini S: Fc-RII/CD23 receptor on circulating human eosinophils. Blood. 1998 Apr 1;91(7):2621-2., URL: http://www.ncbi.nlm.nih.gov/pubmed/9516167,*Ling NR, Stevenson FK, Brown B: Urinary excretion of CD23 antigen in normal individuals and patients with chronic lymphocytic leukaemia (CLL). Clin Exp Immunol. 1991 Dec;86(3):360-6., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=stevenson%,*Yamaoka KA, Arock M, Issaly F, Dugas N, Le Goff L, Kolb JP: Granulocyte macrophage colony stimulating factor induces Fc epsilon RII/CD23 expression on normal human polymorphonuclear neutrophils. Int Immunol. 1996 Apr;8(4):479-90., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=yamaoka%20,*Belleau JT, Gandhi RK, McPherson HM, Lew DB: Research upregulation of CD23 (FcepsilonRII) expression in human airway smooth muscle cells (huASMC) in response to IL-4, GM-CSF, and IL-4/GM-CSF. Clin Mol Allergy. 2005 May 20;3:6., URL: http://www.ncbi.nlm.nih.gov/pubmed/15907205

NCBI and Uniprot Product Information

• NCBI GI #: 119862
• NCBI GeneID: 2208
• UniProt Accession #: P06734
• Molecular Weight: 321
• NCBI Official Full Name: Low affinity immunoglobulin epsilon Fc receptor
• NCBI Official Synonym Full Names: Fc fragment of IgE, low affinity II, receptor for (CD23)
• NCBI Official Symbol: FCER2
• NCBI Official Synonym Symbols: CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor; CD23 antigen; fc-epsilon-RII; lymphocyte IgE receptor; immunoglobulin E-binding factor; C-type lectin domain family 4, member J
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: FCER2
• UniProt Synonym Gene Names: CD23A; CLEC4J; FCE2; IGEBF
• UniProt Entry Name: FCER2_HUMAN

NCBI Description

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).