Mouse anti-Human CD307a Antibody | anti-CD307a antibody

Anti-Hu CD307a Purified

Cat. Num. AAA1802245

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: CD307a; Anti-Hu CD307a Purified; FcRL1; FcRH1; IRTA5; Fc receptor like 1; anti-CD307a antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: E3
• Specificity: The mouse monoclonal antibody E3 recognizes an extracellular epitope of CD307a, a transmembrane glycoprotein expressed predominantly on the surface of mature B cells.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD307a antibody: Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml.
• Immunogen: DNA-immunization followed by a boost with the CD307a transfected cells
• Workshop: HLDA IX
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD307a (E3) purified antibody (5ug/ml, GAM-FITC).)

Flow Cytometry (FC/FACS)

(Separation of human CD307a positive lymphocytes (red-filled) from CD307a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307e (E3) purified antibody (concentration in sample 0,6ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307e (E3) purified antibody (concentration in sample 0,6ug/ml, GAM APC).)

Related Product Information for anti-CD307a antibody

CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.

References

Li H, Borrego F, Nagata S, Tolnay M: Fc receptor-like 5 expression distinguishes two distinct subsets of human circulating tissue-like memory B cells. J Immunol. 2016 May 15;196(10):4064-74., URL: https://pubmed.ncbi.nlm.nih.gov/27076679/
Du X, Nagata S, Ise T, Stetler-Stevenson M, Pastan I: FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins. Blood. 2008 Jan 1;111(1):338-43, URL: https://pubmed.ncbi.nlm.nih.gov/17895404/
Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26, URL: https://www.ncbi.nlm.nih.gov/pubmed/19494275

NCBI and Uniprot Product Information

• NCBI GeneID: 115350
• UniProt Accession #: Q96LA6
• UniProt Protein Name: Fc receptor-like protein 1
• UniProt Gene Name: FCRL1
• UniProt Synonym Gene Names: FCRH1; IFGP1; IRTA5
• UniProt Entry Name: FCRL1_HUMAN