Mouse anti-Human CD160 Monoclonal Antibody | anti-CD160 antibody

Anti-Hu CD160 Alexa Fluor647

• Gene Names: CD160; NK1; BY55; NK28
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD160; Monoclonal Antibody; Anti-Hu CD160 Alexa Fluor647; NK1; NK28; CD160 molecule; anti-CD160 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgM kappa
• Clone Number: BY55
• Specificity: The mouse monoclonal antibody BY55 recognizes an extracellular epitope of CD160, a 27kDa glycoprotein expressed on NK cells, NK-T cells, intestinal intraepithelial lymphocytes, TCR-gamma/delta T cells and a small population of TCR-alpha/beta T cells. The antibody detects both GPI-anchored and transmembrane form of CD160.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD160 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor647
• Immunogen: Human NK cell line YT2C2
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD160 (BY55) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD160 (BY55) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD56 (LT56) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD160 positive CD56 positive lymphocytes (red-filled) from human CD160 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD160 (BY55) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD160 antibody

CD160 is a cell surface glycoprotein of immunoglobulin superfamily, which Functional Application (FUNC)tions as a costimulatory receptor expressed mainly on cytotoxic cell populations and recognizing both classical and non-classical MHC class I molecules. It can form disulfide-linked multimers. Down-modulation of CD160 occurs as a consequence of its proteolytic cleavage and the released soluble form was found to impair the MHC-class I specific cytotoxicity of CD8+ T lymphocytes and NK cells. In contrast to GPI-anchored isoform with broader expression among CD160 positive cells, expression of the transmembrane isoform is restricted to NK cells and is activation-dependent.

References

*Merino J, Ramírez N, Moreno C, Toledo E, Fernández M, Sánchez-Ibarrola A: BY55/CD160 cannot be considered a cytotoxic marker in cytomegalovirus-specific human CD8(+) T cells. Clin Exp Immunol. 2007 Jul;149(1):87-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=merino+j+,*Anumanthan A, Bensussan A, Boumsell L, Christ AD, Blumberg RS, Voss SD, Patel AT, Robertson MJ, Nadler LM, Freeman GJ: Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998 Sep 15;161(6):2780-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=anumantha,*Maiza H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A: A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993 Sep 1;178(3):1121-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/7688788,*Giustiniani J, Bensussan A, Marie-Cardine A: Identification and characterization of a transmembrane isoform of CD160 (CD160-TM), a unique activating receptor selectively expressed upon human NK cell activation. J Immunol. 2009 Jan 1;182(1):63-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/19109136,*Bensussan A, Gluckman E, el Marsafy S, Schiavon V, Mansur IG, Dausset J, Boumsell L, Carosella E: BY55 monoclonal antibody delineates within human cord blood and bone marrow lymphocytes distinct cell subsets mediating cytotoxic activity. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9136-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/8090781

NCBI and Uniprot Product Information

• NCBI GI #: 5901910
• NCBI GeneID: 11126
• NCBI Accession #: NP_008984.1
• NCBI GenBank Nucleotide #: NM_007053.2
• UniProt Accession #: O95971
• Molecular Weight: 19,810 Da
• NCBI Official Full Name: CD160 antigen
• NCBI Official Synonym Full Names: CD160 molecule
• NCBI Official Symbol: CD160
• NCBI Official Synonym Symbols: NK1; BY55; NK28
• NCBI Protein Information: CD160 antigen; CD160-delta Ig; CD160 transmembrane isoform; natural killer cell receptor BY55; natural killer cell receptor, immunoglobulin superfamily member
• UniProt Protein Name: CD160 antigen
• Protein Family: CD160 antigen
• UniProt Gene Name: CD160
• UniProt Synonym Gene Names: BY55
• UniProt Entry Name: BY55_HUMAN

NCBI Description

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor showing broad specificity for both classical and non-classical MHC class I molecules. Ref.3

Subunit structure: Homomultimer; disulfide-linked.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Tissue specificity: Expressed in spleen, peripheral blood, and small intestine. Expression is restricted to functional NK and T cytotoxic lymphocytes.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.