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Flow Cytometry (FC/FACS) (Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD307b (B24) purified antibody (0,5 ug/ml, GAM-FITC).)

Mouse anti-Human CD307b Monoclonal Antibody | anti-CD307b antibody

Anti-Hu CD307b Purified

Gene Names
FCRL2; FCRH2; IFGP4; IRTA4; SPAP1; CD307b; SPAP1A; SPAP1B; SPAP1C
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified by protein-A affinity chromatography
Synonyms
CD307b; Monoclonal Antibody; Anti-Hu CD307b Purified; FcRL2; FcRH2; IFGP4; IRTA4; SPAP1; Fc receptor like 2; anti-CD307b antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
B24
Specificity
The mouse monoclonal antibody B24 recognizes an extracellular epitope of CD307b, a transmembrane glycoprotein expressed mainly in mature and memory B cells.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD307b antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: Recommended dilution: 1-4ug/ml
Immunogen
DNA-immunization followed by a boost with CD307b transfected cells
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD307b (B24) purified antibody (0,5 ug/ml, GAM-FITC).)

Flow Cytometry (FC/FACS) (Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD307b (B24) purified antibody (0,5 ug/ml, GAM-FITC).)
Related Product Information for anti-CD307b antibody
CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia.
References
*Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26, URL: https://www.ncbi.nlm.nih.gov/pubmed/19494275

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,533 Da
NCBI Official Full Name
Fc receptor-like protein 2 isoform 2
NCBI Official Synonym Full Names
Fc receptor-like 2
NCBI Official Symbol
FCRL2
NCBI Official Synonym Symbols
FCRH2; IFGP4; IRTA4; SPAP1; CD307b; SPAP1A; SPAP1B; SPAP1C
NCBI Protein Information
Fc receptor-like protein 2
UniProt Protein Name
Fc receptor-like protein 2
UniProt Gene Name
FCRL2
UniProt Synonym Gene Names
FCRH2; IFGP4; IRTA4; SPAP1; FcR-like protein 2; FcRL2; FcRH2
UniProt Entry Name
FCRL2_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Apr 2009]

Uniprot Description

FCRL2: May have an regulatory role in normal and neoplastic B cell development. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: plasma membrane; integral to membrane

Molecular Function: SH3/SH2 adaptor activity

Biological Process: cell-cell signaling; positive regulation of signal transduction

Research Articles on CD307b

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Product Notes

The CD307b fcrl2 (Catalog #AAA1801813) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD307b Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD307b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the CD307b fcrl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD307b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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