Mouse anti-Human CD305 Monoclonal Antibody | anti-CD305 antibody

Anti-Hu CD305 Alexa Fluor488

• Gene Names: LAIR1; CD305; LAIR-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD305; Monoclonal Antibody; Anti-Hu CD305 Alexa Fluor488; LAIR1; leukocyte associated immunoglobulin like receptor; anti-CD305 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: NKTA255
• Specificity: The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305/LAIR1, a 40kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD305 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor488
• Immunogen: Activated NK cells and CD3- thymocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD305 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD305 antibody

CD305, also known as LAIR1 (leukocyte-associated Ig-like receptor 1), is an inhibitory receptor found on many types of peripheral blood cells. It serves to suppress cell cytotoxicity, activation, proliferation, and differentiation regarding autoantigens via its two intracellular ITIM sites. CD305 belongs to the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family of proteins. It reacts with collagen ligands.

References

*Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A: Defective expression and Functional Application (FUNC)tion of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS One. 2012;7(2):e31903, URL: http://www.ncbi.nlm.nih.gov/pubmed/term=colombo+b,*Poggi A, Tomasello E, Ferrero E, Zocchi MR, Moretta L: p40/LAIR-1 regulates the differentiation of peripheral blood precursors to dendritic cells induced by granulocyte-monocyte colony-stimulating factor. Eur J Immunol. 1998 Jul;28(7):2086-91., URL: http://www.ncbi.nlm.nih.gov/pubmed/9692876,*Poggi A, Tomasello E, Revello V, Nanni L, Costa P, Moretta L: p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes. Int Immunol. 1997 Sep;9(9):1271-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/9310830,*Meyaard L: The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803., URL: http://www.ncbi.nlm.nih.gov/pubmed/18063695,*Son M, Santiago-Schwarz F, Al-Abed Y, Diamond B: C1q limits dendritic cell differentiation and activation by engaging LAIR-1. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3160-7, URL: http://www.ncbi.nlm.nih.gov/pubmed/23093673,*Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A: p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol. 1995 Feb;25(2):369-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/7875198,*Warren HS: The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report. Cell Immunol. 2005 Jul-Aug;236(1-2):17-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/16157321

NCBI and Uniprot Product Information

• NCBI GI #: 612407867
• NCBI GeneID: 3903
• NCBI Accession #: NP_001275952.2
• NCBI GenBank Nucleotide #: NM_001289023.2
• UniProt Accession #: Q6GTX8
• Molecular Weight: 23,049 Da
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform c
• NCBI Official Synonym Full Names: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Symbol: LAIR1
• NCBI Official Synonym Symbols: CD305; LAIR-1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1; leukocyte-associated Ig-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• UniProt Gene Name: LAIR1
• UniProt Synonym Gene Names: CD305; LAIR-1; hLAIR1
• UniProt Entry Name: LAIR1_HUMAN

NCBI Description

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

LAIR-1: an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region called the leukocyte receptor cluster, which contains at least 25 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: immune system process