Human Cathepsin B ELISA Kit | CTSB elisa kit

Human Cathepsin B PicoKine ELISA Kit

Cat. Num. AAA175975

• Gene Names: CTSB; APPS; CPSB
• Reactivity: Human
• Synonyms: Cathepsin B; Human Cathepsin B PicoKine ELISA Kit; cathepsin B; aAmyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CPSB; CTSB; Cysteine protease; CTSB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CTSB
• Sequence Length: 339

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <5pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSB elisa kit

Principle of the Assay: The Picokine™ Human CTSB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CTSB with a 96-well strip plate that is pre-coated with antibody specific for CTSB. The detection antibody is a biotinylated antibody specific for CTSB. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CTSB with immunogen: Expression system for standard: NSO; Immunogen sequence: R18-I339. The kit is analytically validated with ready to use reagents. To measure Human CTSB, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CTSB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CTSB in the sample.

Background/Introduction: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfidelinked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 68067549
• NCBI GeneID: 1508
• UniProt Accession #: P07858; Q503A6; Q96D87; B3KQR5; B3KRR5
• Molecular Weight: 37,822 Da
• NCBI Official Full Name: Cathepsin B
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB
• NCBI Protein Information: cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.1; Motility/polarity/chemotaxis; Protease; Autophagy

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; intracellular membrane-bound organelle; mitochondrion; lysosome; perinuclear region of cytoplasm; extracellular region; melanosome; nucleolus; intracellular

Molecular Function: peptidase activity; collagen binding; protein binding; proteoglycan binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; epithelial cell differentiation; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis