KAT2A recombinant protein

Recombinant KAT2A (GCN5) protein

Cat. Num. AAA388293

• Gene Names: KAT2A; GCN5; hGCN5; GCN5L2; PCAF-b
• Synonyms: KAT2A; Recombinant KAT2A (GCN5) protein; Lysine Acetyltransferase 2A; Histone Acetyltransferase GCN5; GCN5L2; PCAF-b; KAT2A recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant GCN5 protein is supplied at a concentration of 0.75ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant KAT2A (GCN5) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Assay Conditions: 0.5ug Histone H3.1 was incubated with 0ug (-), 0.1ug (+), 0.2ug (++), 0.4ug (+++) GCN5 protein in a 30ul reaction system containing 50mM Tris-HCl pH 8.0, 0.1mM EDTA, 50 ng/ul BSA, 1mM TCEP and 20uM Acetyl-CoA for 2 hours at room temperature. All samples were run on a 12.5% SDS-PAGE gel, and products were detected by Western blot. H3ac (pan-acetyl) antibody was used to recognize acetylated histone H3.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant KAT2A (GCN5) protein, SDS-PAGE gel Recombinant KAT2A (GCN5) protein was run on a 10% SDS-PAGE gel and stained with Coomassie BlueMW: 95.6 kDaPurity: > 85%)

Western Blot (WB)

(Recombinant KAT2A (GCN5) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) GCN5 catalytic domain in 30 ul reaction system for 2 hours at room temperature. Products were detected by Western blot. H3ac (pan-acetyl) antibody was used to recognize acetylated histone H3.)

Related Product Information for KAT2A recombinant protein

Short Description: Recombinant KAT2A (GCN5) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_066564.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 95.6 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: GCN5 (General Control Of Amino Acid Synthesis Protein 5, also known as KAT2A, Lysine Acetyltransferase 2A) is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also acts as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT- independent manner. KAT2A (GCN5) has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. It also acetylates non-histone proteins, such as CEBPB. KAT2A (GCN5) is a core component of the ATAC complex, which is a complex with histone acetyltransferase activity on histones H3 and H4.

Product Categories/Family for KAT2A recombinant protein

Recombinant Proteins; Histone Acetyltransferase (HAT)

NCBI and Uniprot Product Information

• NCBI GI #: 153791535
• NCBI GeneID: 2648
• NCBI Accession #: NP_066564.2
• NCBI GenBank Nucleotide #: NM_021078.2
• UniProt Accession #: Q92830; Q8N1A2; Q9UCW1
• Molecular Weight: 93,926 Da
• NCBI Official Full Name: histone acetyltransferase KAT2A
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 2A
• NCBI Official Symbol: KAT2A
• NCBI Official Synonym Symbols: GCN5; hGCN5; GCN5L2; PCAF-b
• NCBI Protein Information: histone acetyltransferase KAT2A; STAF97; hsGCN5; lysine acetyltransferase 2A; histone acetyltransferase GCN5; general control of amino acid synthesis protein 5-like 2; General control of amino acid synthesis, yeast, homolog-like 2; GCN5 (general control o
• UniProt Protein Name: Histone acetyltransferase KAT2A
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: KAT2A
• UniProt Synonym Gene Names: GCN5; GCN5L2; HGCN5; HsGCN5
• UniProt Entry Name: KAT2A_HUMAN

NCBI Description

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]

Uniprot Description

GCN5: an ubiquitous histone acetyltransferase that promotes transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Interacts with P300, CBP, ADA2, and is a component of the TFTC-HAT complex. Two splice variant isoforms have been described.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; extracellular space

Molecular Function: protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; chromatin binding; H3 histone acetyltransferase activity

Biological Process: transcription from RNA polymerase II promoter; somitogenesis; establishment and/or maintenance of chromatin architecture; viral reproduction; in utero embryonic development; multicellular organism growth; regulation of protein stability; metencephalon development; histone deubiquitination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; cell proliferation; telencephalon development; midbrain development; neural tube closure