SDS-Page
(Recombinant BRPF3 (576-701) protein gel. BRPF3 (576-701) protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
BRPF3 recombinant protein
Recombinant BRPF3 (576-701) protein
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
BRPF3; Recombinant BRPF3 (576-701) protein; Bromodomain And PHD Finger Containing 3; KIAA1286; BRPF3 recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant BRPF3 (576-701) protein expressed in E Coli cells and is provided in 25mM Tris pH7.4, 150mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant BRPF3 (576-701) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRPF3 (576-701) protein gel. BRPF3 (576-701) protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant BRPF3 (576-701) protein gel. BRPF3 (576-701) protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(Recombinant BRPF1 (576-701) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (576-701) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(Recombinant BRPF1 (576-701) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (576-701) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for BRPF3 recombinant protein
Short Description: The peptide corresponding to amino acids 576-701 that contains the bromodomain sequences of BRPF3 (accession number NM_015695.2) was expressed in E Coli and contains an N-terminal His-Tag and C-terminal DYKDDDDK-Tag with an observed molecular weight of 19.7 kDa. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: BRPF3 (Bromodomain and PHD finger containing 3) is a component of the MOZ/MORF histone acetyltransferase (HAT) complex. The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues can be recognized by bromodomain. The bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. There is less study on its function now.
Background: BRPF3 (Bromodomain and PHD finger containing 3) is a component of the MOZ/MORF histone acetyltransferase (HAT) complex. The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues can be recognized by bromodomain. The bromodomains serve as "readers" of histone acetylation marks regulating the transcription of target promoters. There is less study on its function now.
Product Categories/Family for BRPF3 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 19.7kDa
Protein Family
Similar Products
Product Notes
The BRPF3 (Catalog #AAA388198) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRPF3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
