Western Blot (WB)
(Mouse Prpf4b Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Prpf4b antibody detected the Prpf4b protein (arrow).)
Rabbit anti-Mouse Prpf4b Polyclonal Antibody | anti-PRPF4B antibody
Prpf4b, NT (Prpf4b, Prp4h, Cbp143, Prp4k, Prp4m, Prpk, Serine/threonine-protein kinase PRP4 homolog, PRP4 pre-mRNA-processing factor 4 homolog, Pre-mRNA protein kinase)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Mouse Prpf4b Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Prpf4b antibody detected the Prpf4b protein (arrow).)
Western Blot (WB)
(Mouse Prpf4b Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Prpf4b antibody detected the Prpf4b protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]
Uniprot Description
PRP4: a kinase of the DYRK family associated with the spliceosome and the kinetochore. It binds pre-mRNA splicing factors SFRS8, PRP6 and pinin. It is an essential kinase that associates with both the U5 snRNP and the N-CoR deacetylase complexes. It may help coordinate pre-mRNA splicing with chromatin remodeling and transcriptional regulation. PRP4 has recently been shown to be a kinetochore component that is necessary to establish a functional spindle assembly checkpoint, and is necessary for recruitment or maintenance of the checkpoint proteins RPS27, MAD1, and MAD2 at the kinetochores.
Protein type: Protein kinase, CMGC; Spliceosome; RNA splicing; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; DYRK family; PRP4 subfamily
Chromosomal Location of Human Ortholog: 6p25.2
Cellular Component: nucleoplasm; chromosome
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; protein amino acid phosphorylation
Research Articles on PRPF4B
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Product Notes
The PRPF4B prpf4b (Catalog #AAA6009006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prpf4b, NT (Prpf4b, Prp4h, Cbp143, Prp4k, Prp4m, Prpk, Serine/threonine-protein kinase PRP4 homolog, PRP4 pre-mRNA-processing factor 4 homolog, Pre-mRNA protein kinase) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prpf4b can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PRPF4B prpf4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prpf4b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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